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杨建华,李长德,马杰,$2.胚胎干细胞移植在中枢神经系统损伤小鼠中的应用[J].中国康复医学杂志,2005,20(8):569~
胚胎干细胞移植在中枢神经系统损伤小鼠中的应用    点此下载全文
杨建华  李长德  马杰  $2
[1]佳木斯大学附属第一医院骨一科,154002 [2]上海第二医科大学附属新华医院神经外科,154002 [3]上海发育生物学研究中心,154002
基金项目:国家自然科学基金资助(30470599/C030307)
DOI:
摘要点击次数: 1980
全文下载次数: 115
摘要:
      目的:观察经诱导的胚胎干细胞(ES)移植在小鼠脊髓损伤和小鼠缺氧缺血性脑病中的行活和迁移。方法:利用小鼠脊髓损伤(SCI)和缺氧缺血性脑病的模型(HIE),将小鼠胚胎T细胞体外诱导分化为ES细胞的衍生细胞后,注射到脊髓损伤和缺氧缺血性脑病的小鼠体内。移植后观察12周,应用酶学的方法检测Lae-Z标记的经诱导的ES细胞在脊髓和脑内的存活和迁移情况。结果:ES细胞在中枢冲经系统损伤区能存活,并进行长距离的迁移,并与周围组织整合。结论:经诱导的胚胎干细胞能在宿主损伤脑和脊髓中存活、迁移,且脑内迁移较脊髓内迁移明显。
关键词:中枢神经系统损伤 胚胎干细胞 移植 小鼠
A study of following embryonic stem cells transplantation to central nervous system in spinal injury mouse    Download Fulltext
Yang JianHua;Li ChangDe;Ma Jie;Wang Yu
Fund Project:
Abstract:
      Objective: To observe the survival and migration by transplantation of embryonic stem cells in SCI andHIE in mouse. Method: Labeled XY with Lac- Z marked gene, of mouse ES cell was cultured and induced to dif-ferentiate by specialized factors in vitro. ES cell- derived cells were planted into vertebral canal around injuredspinal cord and cerebral ventricle of HIE model. Model animals were observe at 8 weeks after transplantation.Spinal cord and brain tissue were harvested for enzymology staining to detect whether the Lac- Z- labeled geneESc express. Result: ES cell- derived cells could survive and migrate in the injured region of central nervous sys-tem in a long distance, and communications with peritissue.Conclusion:ES cell- derived cells could survive and mi-grate in central nervous system injured region, brian migration was more apparent in comparision with spinal migra-tion.
Keywords:central nervous system injury  embryonic stem cells  transplantation  mouse
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