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卢昌均,陆兵勋,王立新,尹瑞雪,刘忆星.缺血再灌注损伤大鼠脑内GFAP的变化及通心络的影响[J].中国康复医学杂志,2006,21(3):234~236
缺血再灌注损伤大鼠脑内GFAP的变化及通心络的影响    点此下载全文
卢昌均  陆兵勋  王立新  尹瑞雪  刘忆星
南方医科大学南方医院神经内科,广州 510515
基金项目:国家自然科学基金资助项目(30400612)
DOI:
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摘要:
      目的:探讨胶质纤维酸性蛋白(GFAP)在脑缺血再灌注损伤(MCAO)后星形胶质细胞的活化增殖情况及通心络对其影响.方法:采用大鼠缺血再灌注损伤(MCAO)模型,应用免疫组织化学方法观察缺血后3d、7d、14d以及21d缺血侧室管膜及室管膜下区(SVZ)、海马齿状回(SGZ)GFAP的变化.给予模型大鼠通心络灌胃,观察神经干细胞增殖分化的变化.结果:GFAP阳性细胞随缺血再灌注时间的延长,荧光强度值增加,第7天、第14天、第21天组与假手术组比较,差异具有显著性意义(P<0.05).造模后通心络组BrdU阳性细胞荧光强度值和BrdU+GFAP免疫双标荧光强度值均高于脑缺血再灌注模型组,差异显著(P<0.05).结论:大鼠缺血再灌注损伤后可引起其缺血侧SVZ、SGZ区星形胶质细胞反应和增殖:而通心络可显著增加MCAO大鼠神经干细胞增殖分化能力.
关键词:脑缺血 胶质纤维酸性蛋白 大鼠 通心络
The activation of GFAP after middle cerebral artery occlusion in rats and treatment with Tongxinluo    Download Fulltext
LU Changjun  LU Bingxun  WANG Lixin  et al
Dept. of Neurology, Nanfan Hospital, Southern Medical University, Guangzhou, 510515
Fund Project:
Abstract:
      Objective: To investigate the activation of glial fibrillary acidic protein(GFAP) after middle cerebral artery occlusion in rats and treatment with Tongxinluo.Method:Ischemia was induced by temporary middle cerebral artery occlusion(MCAO).In ependyma and subependymal zone(SVZ)?Hippocampus dentate gyrus zone(SGZ) of rats at 3?7?14 and 21 days after MCAO, the numbers of GFAP were detected by immunohistochemistry which were treated with Tongxinluo.Result:Compared with the sham operation group, GFAP was significantly increased in the MCAO on the day 7?14 and 21(P<0.05). Immunofluorescene intensity of BrdU+GFAP had significantly increased in SVZ.After the treatment of Tongxinluo, the number of BrdU and immunofluorescene intensity of BrdU+GFAP were significantly increased as compared with the MCAO group(P<0.05).Conclusion: This study demonstrates that the focal cerebral ischemia in the rat results in a rapid response, a process often referred to as reactive GFAP, from resident astrocytes of the SVZ and SGZ to the side of ischemia;Tongxinluo enhances the ability of the differentiation and proliferation of neural stem cell in MCAO.
Keywords:cerebral ischemia  glial fibrillary acidic protein  rat  Tongxinluo
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