饮食和运动干预对2型糖尿病大鼠骨骼肌Cu/ZnSOD mRNA表达的影响

关键词：运动,饮食,2型糖尿病,铜锌超氧化物歧化酶,基因表达

Keywords:Cu/Zn-Superoxide dismutase,exercise training,diet,diabetic rat,gene expression

摘要：

      目的:探讨饮食和运动对糖尿病大鼠骨骼肌细胞Cu/ZnSODmRNA表达及其血浆酶活性的影响。方法:雄性Sprague-Dawley大鼠24只采用高糖高脂饮食后腹腔注射小剂量链脲霉素,建立糖尿病模型,随机分为4组:糖尿病高脂饲料非运动组(DFN,n=6),糖尿病常规饲料非运动组(DRN,n=6),糖尿病常规饲料低强度运动组(DRL,n=6),糖尿病常规饲料高强度运动组(DRH,n=6)。活动平板进行耐力训练8周,测定骨骼肌Cu/ZnSODmRNA和血浆Cu/ZnSOD活性的变化。结果:两运动组骨骼肌Cu/ZnSODmRNA表达显著高于DFN组和DRN组,两运动组之间无显著差异。两运动组血浆Cu/ZnSOD活性显著高于DFN组和DRN组,DRN组血浆Cu/ZnSOD活性显著高于DFN组。结论:饮食调整加运动训练可以促进糖尿病大鼠骨骼肌Cu/ZnSODmRNA表达,提高Cu/ZnSOD酶活性,而运动强度对此无显著影响。

Abstract:

      Objective:To explore the effects of diet and exercise training on gene expression of skeletal muscle and plasma activities in Cu/ZnSOD in type 2 diabetic rats.Method:Twenty-four male Sprague-Dawley rats were fed on high sucrose diet with high fat for 4 weeks and then injected with low dose of streptozotocin to establish type the 2 diabetic model. The diabetic rats were divided randomly into four groups: high sucrose and fat diet without exercise(DFN),regular diet without exercise(DRN),regular diet with 75%VO2max exercise(DRH). The animals with 55%VO2max exercise(DRL). The animals with exercise training were forced to running on motorized treadmill for 8 weeks. The mRNA expression of skeletal muscle and plasma activity in Cu/ZnSOD were measured.Result:The plasma Cu/ZnSOD activities in the two exercise training groups were significantly higher than that in the DFN and DRN groups,in which plasma Cu/ZnSOD activities in DRN group were remarkably higher than that in DFN group. The expression of Cu/ZnSOD mRNA in skeletal muscle increased significantly in the two exercise training groups,in which there were no significant differences between the two exercise training groups. Conclusion:Diet plus exercise training can enhance mRNA expression of skeletal muscle and plasma activity in Cu/ZnSOD which is not associated with the intensity of exercise training.