| 吴永超,郑启新,杜靖远,吴斌.甲泼尼龙抑制神经干细胞并诱导其凋亡的研究[J].中国康复医学杂志,2006,21(9):776~ |
| 甲泼尼龙抑制神经干细胞并诱导其凋亡的研究 点此下载全文 |
| 吴永超 郑启新 杜靖远 吴斌 |
| 华中科技大学同济医学院附属协和医院骨科,武汉430022 |
| 基金项目：国家自然科学基金资助项目（30500511） |
| DOI： |
| 摘要点击次数: 1920 |
| 全文下载次数: 1723 |
| 摘要： |
| 目的：研究甲泼尼龙对体外培养的神经干细胞的直接作用，为有效的结合这两种治疗方法提供依据和指导。方法：体外培养神经干细胞，加入MP培养12h、24h和48h后，用细胞活性检测试剂CCK-8，检测其对神经干细胞活性的影响，用Hoeehst 33258染色观察细胞凋亡，用Annexin V／PI双染色法检测神经干细胞凋亡比率。结果：甲泼尼龙对体外培养的活性有抑制作用，而且可以诱导体外培养的神经干细胞的发生凋亡。结论：甲泼尼龙不利于神经干细胞的生长，不宜在进行甲泼尼龙冲击疗法的同时进行神经干细胞移植。 |
| 关键词：甲泼尼龙 神经干细胞 凋亡 |
| Methylprednisolone introduce inhibition and apoptosis to neural stem cells in vitro Download Fulltext |
| WU Yongchao ZHENG Qixin DU Jingyuan et al. |
| Dept. of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022 |
| Fund Project: |
| Abstract: |
| Objective:To study the direct effect of methylprednisolone to neural stem cells in vitro, and provide direction for the combined usage of these two therapy methods.Method:Neural stem cells were obtained and cultured in vitro. Methylprednisolone(250ng/ml) was added to the culture media for 12h,24h and 48h. The activity of NSC was tested with CCK-8(cell counting kit). NSCs were dyed with Hoechst 33258 and Annexin V-FITC/PI apoptosis kit. The apoptosis rates were tested with flow cytometry. Result:Methylprednisolone inhibited the activity of neural stem cells in vitro, and also promoted apoptosis of neural stem cells.Conclusion:Methylprednisolone provides a adverse environment for neural stem cells, so combined usage of methylprednisolone and neural stem cells should be avoided. |
| Keywords:methylprednisolone neural stem cell apoptosis |
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