神经生长因子对大鼠脑缺血再灌注损伤的保护作用

关键词：神经生长因子 脑缺血再灌注损伤 神经评分 一氧化氮合酶 大鼠

Keywords:nerve growth factor  , brain ischemia-reperfusion injury  neurological grades  nitric oxide synthase  rats

摘要：

      目的：探讨神经生长因子（NGF）对大鼠脑缺血再灌注损伤的保护作用及其机制。方法：采用大脑中动脉内栓线阻断法（MCAO）造成大鼠局灶性损伤模型，同时给予NGF治疗。观测神经评分改变，计算脑组织梗死灶，测定脑组织含水量以及一氧化氮合酶（NOS）活动的变化。结果：NGF治疗组神经评分明显降低（P〈0.05）；与脑缺血再灌注组比较，NGF治疗组脑缺血梗死区缩小，脑组织含水量和NOS活性明显降低（P〈0．05）。结论：NGF具有保护脑缺血再灌注损伤的作用，可能与抑制NOS的活性有关。

Abstract:

      Objective:To study the protective effects and mechanism of nerve growth factor(NGF) on acute brain lesion induced by ischemia-reperfusion in rats.Method:The focal ischemia-reperfusion modal was made by thread embolism of middle cerebral artery,after treatment of NGF.The neurological grades were observed,the infarct size,the brain water content and the activity of nitric oxide synthase(NOS) were measured.Result:The neurological grades were significantly lowered after treatment compared with ischemia-reperfusion group(P<0.05),the infarct size,the brain water content and the activity of NOS in NGF treatment group were obviously lower than that in the ischemia-reperfusion group(P<0.05).Conclusion:NGF can accelerate the nervous function recovery of the ischemia-reperfusion injured rats,the mechanism is related to NGF prohibits NO production and play a role on amelioration of the brain ischemia-reperfusion injury.