雌兔去势对膝关节软骨及关节液和血清中透明质酸的影响

关键词：骨关节炎 透明质酸 雌激素 膝关节软骨 关节液

Keywords:osteoarthritis  hyaluronic acid  estrogen  knee cartilage  joint synovia

摘要：

      目的：观察雌激素水平变化对兔膝关节软骨及关节液和血清中透明质酸的影响。方法：56只雌兔随机分为4组，A组正常对照，B组关节固定，为OA模型组，C组切除卵巢后不补充雌激素，D组切除卵巢后补充雌激素，分别于第2、4、6、8周抽血和关节液，测定HA水平，并取右股骨内侧髁软骨组织进行光镜和电镜观察。结果：B、C组关节液HA均下降，血清HA均上升，与A组比较差异有显著性意义（P〈0．05），D组关节液和血清HA与A组比较均无显著性差异（P〉0．05）；标本观察显示，B、C两组关节软骨均有OA改变，但B组重于C组，D组软骨退变程度较C组轻。结论：雌激素水平下降，可致软骨退变．关节液及血清HA水平发生变化，其比值下降明显。雌激素水平下降可能关系到OA的发生发展，关节液HA及血清HA比值下降，血清HA升高可能是OA的重要指标。

Abstract:

      Objective:To explore the changes of knee cartilage,and the changes of hyaluronate acid(HA) in serum and joint synovia and HA ratio in osteoarthritis(OA) and ovariectomized rabbits.Method:Fifty six female rabbits were randomly divided into four groups:8 rabbits as control(Group A) ,16 rabbits' knee fixed as an OA model(Group B) ,16 rabbits with ovariectomy(Group C) and 16 rabbits ovariectomy with estrogen(Group D) .HA in serum and joint synovia were measured by radio-immunity technique at the 2nd,4th,6th,8th week after ovariectomization,partial cartilage on internal condyle of right femur were observed by light and electron-microscopy.Result:HA of joint synovia decreased and that of serum increased in Group B and C,there was difference compared with Group A(P<0.05) ;and there was no significant change in Group D in all time points(P>0.05) .Under microscope the cartilage specimens showed that there were OA changes in Group B and C,but in Group B that was more severe than in Group C;and in Group D that was lighter than in Group C.Conclusion:The estrogen deficiency could be related to OA development.HA in serum and joint synovia changed,and HA ratio decreased significantly.There could be important markers of OA and have relative value in clinic for diagnosis,prevention and treatment of OA.