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曹师承,孙黎光,赵 刚,刘慧莉,张 合.有氧运动对大鼠骨骼肌mTOR活性与蛋白表达的影响[J].中国康复医学杂志,2008,23(1):34~36
有氧运动对大鼠骨骼肌mTOR活性与蛋白表达的影响    点此下载全文
曹师承  孙黎光  赵 刚  刘慧莉  张 合
中国医科大学基础医学院生物化学与分子生物学教研室,沈阳,110001;中国医科大学基础医学院生物化学与分子生物学教研室,沈阳,110001
基金项目:辽宁省教育厅高等学校科研基金资助项目(2004D244)
DOI:
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摘要:
      目的:观察有氧运动对大鼠骨骼肌哺乳动物雷帕霉素靶蛋白(mTOR)蛋白总量(t-mTOR)及磷酸化mTOR(p-mTOR)与磷酸化的核糖体蛋白S6激酶 (p-P70S6K)的影响。方法:SD大鼠随机分为对照组和运动组。运动组1.5 h/d训练,共7周。运动结束后分别在24h或48h取材,测定葡萄糖和胰岛素浓度;Western blot法检测骨骼肌t-mTOR蛋白表达、p-mTOR和p-P70S6K磷酸化水平。结果:与对照组比较,运动组胰岛素浓度降低;各运动组t-mTOR升高;p-mTOR和p-P70S6K活性增高显著。结论:运动能提高大鼠骨骼肌对胰岛素的敏感性,改善mTOR与P70S6K磷酸化及mTOR蛋白表达。
关键词:哺乳动物雷帕霉素靶蛋白  运动  骨骼肌  核糖体蛋白S6激酶
Effects of aerobic exercises on mTOR phosphorylation and protein expression in skeletal muscle of rats    Download Fulltext
Department of Biochemistry and Molecular Biology, Shenyang, 110001;Department of Biochemistry and Molecular Biology, Shenyang, 110001
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Abstract:
      Objective:To investigate the effects of exercises on mTOR and P70S6K phosphorylation and protein expression of mTOR. Method: Male SD rats were randomly divided into control group and trained group. The trained rats were submitted to 1.5h of exercises daily and had a fragment of their excised gastrocnemius muscle, 24h or 48h after the last training session. The training lasted for 7 weeks.The changes in the expressions of mTOR and p-mTOR and p-P70S6K were determined by Western blotting. The serum levels of glucose and insulin were measured. Result: Glucose tolerance test diplayed blood insulin concentration decreased with exercises training. Exercises led to a marked increase in p-mTOR and p-P70S6K of trained group as compared with that of controls, and increased mTOR protein expression of training 1.5h/d, 24h and 48h after the last training session.Conclusion:Exercises could elevate skeletal muscle responsiveness to insulin, improve the p-mTOR and p-P70S6K and protein expression of mTOR.
Keywords:mammalian target of rapamycin  exercises  skeletal muscle  ribosomal protein S6 kinas
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