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黄艳君,罗 勇.电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑内神经干细胞增殖的影响[J].中国康复医学杂志,2008,23(3):211~215
电刺激小脑顶核对大鼠局灶脑缺血再灌注后脑内神经干细胞增殖的影响    点此下载全文
黄艳君  罗 勇
重庆医科大学附属第一医院神经内科,400016
基金项目:重庆市科委攻关项目(渝科发计字[2003]43号文,2003-7)、重庆市卫生局科研项目(渝卫科教[2005]31号文,编号:2005-2-179)
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摘要:
      摘要 目的:研究电刺激小脑顶核(FNS)对成年大鼠局灶脑缺血再灌注后脑内不同部位、不同时间点神经干细胞增殖的变化规律,探讨FNS治疗缺血性脑损伤的作用机制。方法:雄性Wistar大鼠随机分为正常组(NC组)、假手术组(SC组)、局灶脑缺血再灌注组(I/R组)、局灶脑缺血再灌注+小脑顶核假刺激组(I/RFs组)、局灶脑缺血再灌注+小脑顶核刺激组(I/RF组),每组根据再灌注时间的不同又分为第1、3、7、14、21、28天6个亚组(n=6)。缺血时间均为1h/再灌注,于再灌注后立即刺激对侧小脑顶核1h。采用线栓法制备大鼠右侧大脑中动脉局灶脑缺血再灌注模型。结果:局灶脑缺血再灌注后不同时间点Brdu阳性细胞在缺血侧侧脑室区、海马齿状回的表达均有增加,呈单峰变化趋势,第1天开始增加(P<0.01),第7天达到高峰(P<0.01),第14天后下降(P<0.01);第21、28天时已明显下降(P<0.05),而FNS后,Brdu阳性细胞数量在缺血/再灌注基础上增加更加明显(P<0.05,P<0.01),第7天达到高峰(P<0.01),峰值更高,第14天后仍维持在较高水平(P<0.01),第21天后逐步下降(P<0.05)、第28天已明显下降;且引起整个侧脑室和海马齿状回、颗粒下层、锥体细胞层细胞增殖。Brdu阳性细胞有明显形态改变。结论:FNS可促进局灶脑缺血再灌注后脑内侧脑室和海马Brdu阳性细胞的增殖,提示这种作用可能是FNS治疗缺血性脑损伤的重要作用机制之一。 关键词 局灶脑缺血再灌注;神经干细胞;电刺激,小脑顶核 中图分类号:R364.1,R454.1 文献标识码:A 文章编号:1001-1242(2008)-03-0211-05
关键词:局灶脑缺血再灌注  神经干细胞  电刺激,小脑顶核
Effect of electrical stimulating to fastigial nucleus on proliferation of neural stem cell in brain of adult rat after focal cerebral ischemia/reperfusion    Download Fulltext
Dept. of Neurology, the First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing Key Laboratory of Neurology, Chongqing, 400016
Fund Project:
Abstract:
      Objective:To study the influence of electrical stimulating to fastigial nucleus on the proliferation regularity of expression of Brdu in adult rat brain after focal cerebral ischemia and to explore the mechanism of fastigial nucleus electrical stimulation(FNS) in treating ischemic brain injury. Method:The rats were randomly divided into five groups: normal control group (NC group), sham operation control group(SC group), ischemia/reperfusion group (I/R group), ischemia/reperfusion treated with sham FNS group(I/R Fs group), and ischemia/reperfusion treated with FNS group (I/RF group), each group contained the 1st, 3rd, 7th, 14th, 21st, 28th d six time points, at each point n=6. Animal models of focal cerebral ischemia/reperfusion were made by filament occlusion of right middle cerebral artery.Result:After focal cerebral ischemia/reperfusion, the number of Brdu positive cells increased at each time point, reached small peak value at the 7th d (P<0.01). With FNS, the number of Brdu positive cells increased more strikingly at each time point(P<0.05, P<0.01), reached higher peak value at the 7th d (P<0.01).Conclusion:Fastigial nucleus electrical stimulation can promote the proliferation of Brdu positive cells in some brain regions after focal cerebral ischemia/reperfusion, which might be one of the important mechanisms of fastigial nucleus stimulation in treating ischemic brain injury.
Keywords:focal cerebral ischemia/reperfusion  neural stem cell  electrical stimulation  fastigial nucleus
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