复合振动预防去势大鼠骨质疏松的实验研究

关键词：骨质疏松  复合振动  大鼠

Keywords:osteoporosis  compound vibration  rat

摘要：

      摘要 目的：观察低强度复合振动对去势SD雌性大鼠生长期骨密度的影响。方法：4月龄SD雌性未育大鼠48只随机分为正常对照组（假手术组）、去势对照组以及4个去势振动组，每组8只。两对照组不作振动干预，4个振动组分别接振不同参数振动，干预时间13周。比较大鼠体重，腰椎、股骨骨密度，骨转换标志物（血清骨钙素、碱性磷酸酶、非前列腺酸性磷酸酶、Ⅰ型胶原C端肽）值。结果：实验开始后除正常对照组外，其余各组体重均明显增加。各组股骨骨密度均增加，差异无显著性意义，振动3、4组与正常对照组较去势对照组腰椎骨密度显著性增加。骨转换标志物各组差异无显著性意义。结论：一定的复合振动可以增加去势SD大鼠腰椎骨密度，具有潜在的预防骨质疏松作用。 关键词 骨质疏松;复合振动;大鼠 中图分类号：R331,R49 文献标识码：A 文章编号：1001-1242(2008)-04-0325-05

Abstract:

      Objective:To observe the effect of compound vibration on bone mineral density(BMD) of ovariectomized rats. Method: Fourty eight SD rats of 4-months old were randomly divided into 6 groups(n=8): normal control group(NC, sham-operation group), ovariectomized control group(OC), and four ovariectomized plus vibration groups(OV): group 1 (OV1,35Hz－45Hz, 0.05g－0.1g), group 2(OV2,35Hz－45Hz, 0.12g－0.21g), group 3 (OV3,45Hz－55Hz, 0.05g－0.1g) and group 4(OV4,45Hz－55Hz, 0.12g－0.21g). The experiment time was 13 weeks. The weight, BMD and serum bone-turn markers were compared among groups respectively.Result:From the 1st month, weight of all ovariectomized groups were greater than NC group(P=0.000). Femur BMD of each group was increased but with no statistical difference among groups. Only OV3 (P=0.005), and OV4(P=0.001) improved the lumbar BMD compared with OC group, and reached the level of NC. Bone-turn marker failed to show a significant difference among all the groups.Conclusion:The compound vibration with the parameter of 45Hz-55Hz, 0.05g－0.1g and 0.12g－0.21g could maintain or improve lumbar BMD of ovariectomized growing rats. The vibration treatment may be used in prevention of osteoporosis.