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董 锋,林建华,吴朝阳.骨髓间质干细胞经静脉注射移植对大鼠脊髓损伤后BDNF、NGF mRNA表达的影响[J].中国康复医学杂志,2008,23(5):416~419
骨髓间质干细胞经静脉注射移植对大鼠脊髓损伤后BDNF、NGF mRNA表达的影响    点此下载全文
董 锋  林建华  吴朝阳
福建医科大学附属第一医院骨科,福建省福州市台江区茶中路20号,350005
基金项目:教育部科学技术研究重点项目(01058);福建省科技厅重点项目(2002Y014);福州市科技局项目(2002-14)
DOI:
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摘要:
      目的:研究大鼠骨髓间质干细胞(rat mesenchymal stem cells,rMSCs)静脉注射移植对脊髓损伤(spinal cord injury,SCI)后脑源性神经营养因子(brain derived neurotrophic factor, BDNF)、神经生长因子(nerve growth factor, NGF)表达的影响,并探讨骨髓间质干细胞移植治疗大鼠脊髓损伤的机制。方法:运用改良Allen法制备大鼠T10脊髓外伤性截瘫模型,假手术组6只,损伤组84只随机分为对照组和rMSCs移植组。rMSCs组、假手术组接受rMSCs单细胞悬液1ml(1×106个rMSCs)自大鼠尾静脉缓慢注射移植,对照组静脉注射PBS1ml。移植后3h、6h、12h、24h、3d、7d、14d,应用RT-PCR方法检测损伤大鼠脊髓BDNF、NGF mRNA表达变化情况。结果:对照组和rMSCs移植组损伤脊髓BDNF、NGFmRNA表达较假手术组有明显增加(P<0.05);rMSCs 移植组与对照组比较BDNF、NGFmRNA表达增加更为明显 (P<0.05)。结论:脊髓损伤后损伤脊髓局部的BDNF、NGF表达增加,rMSCs静脉注射移植后能促进损伤脊髓局部的BDNF、NGF更进一步的表达,这可能是促进大鼠神经结构及神经功能恢复的因素之一。
关键词:脊髓损伤  骨髓间质干细胞  脑源性神经营养因子  神经生长因子  静脉移植
Influence of intravenous administration of bone marrow mesenchymal stem cells on expression of BDNF,NGF mRNA after spinal cord injury in rats    Download Fulltext
Dept. of Orthopaedics, The First Hospital of Fujian Medical University, Fuzhou, 350005
Fund Project:
Abstract:
      Objective:To explore the Influence of intravenous administration of bone marrow mesenchymal stem cells on expression of brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) mRNA after spinal cord injury in rats and to study possible mechanism of intravenous administration of bone marrow mesenchymal stem cells after spinal cord injury in adult rats. Method:Rats were subjected to a weight driven implant injury. There are 6 in the non-injuried group and 84 rats in the injuried group. Then the injuried group was randomly divided into transplantation group and control group. There were 7 time points in each group, n=6 in each point.rMSCs or PBS were infused into ta-il vein at 10 minutes after injury. Result:Expression of BDNF、NGF mRNA in the rMSCs group and in the control group was higher than that in non-injured spinal cord sections(P<0.05). Compared with the control s group, expression of BDNF、NGF mRNA in the rMSC group was higher significantly(P<0.05).Conclusion:After spinal cord injury in rats, expression of BDNF、NGF was improved and advanced improved via intravenous administration of bone marrow mesenchymal stem cells.Maybe it is one of the mechanism of promoting restoration of injured spinal cord and improving motor function.
Keywords:spinal cord injury  bone marrow mesenchymal stem cells  brainderived neurotrophic factor  nerve growth factor  intravenous transplantation
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