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原睿智,张新定,冯 伟,韩 广,韩彦明,周旺宁,程得均,冯广才.促红细胞生成素对大鼠创伤性脑损伤保护作用的研究[J].中国康复医学杂志,2008,23(11):1025~1027
促红细胞生成素对大鼠创伤性脑损伤保护作用的研究    点此下载全文
原睿智  张新定  冯 伟  韩 广  韩彦明  周旺宁  程得均  冯广才
兰州大学第二医院神经外科,兰州市,730030
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DOI:
摘要点击次数: 2099
全文下载次数: 2081
摘要:
      目的:探讨促红细胞生成素(EPO)对大鼠创伤性脑损伤(TBI)后血脑屏障、脑水肿、脑损伤体积和血清神经元特异性烯醇化酶(NSE)的影响。 方法:应用Feeney法制作大鼠创伤性脑损伤实验模型。选取72只成年Wistar大鼠,随机分成假手术组(24只)、对照组(24只)和EPO治疗组(24只)。制模成功48h后通过测定脑组织伊文思兰(EB)含量观察血脑屏障(BBB)的破坏程度、干湿重法测脑水含量、TTC染色法测定脑损伤体积。24h时放免法测定血清NSE含量。结果:EPO治疗组BBB破坏程度(67.3±13.1μg/g)与对照组(182.8±15.9μg/g)相比显著减轻(P<0.01)。EPO治疗组脑水肿(80.6%±0.2%)较对照组(91.8%±0.6%)明显减轻(P<0.01)。EPO治疗组脑损伤体积(17.9±4.0mm3)较对照组(37.7±3.8mm3)明显变小(P<0.01)。EPO组血清NSE水平(6.28±3.37ng/ml)也较对照组(10.02±1.50ng/ml)显著降低(P<0.05)。结论:EPO能维持血脑屏障的完整性,缩小脑损伤体积,减轻脑水肿和神经元的损害。
关键词:促红细胞生成素  创伤性脑损伤  血脑屏障  脑水肿  神经元特异性烯醇化酶
The study of neuroprotection about erythropoietin on traumatic brain injury in rats    Download Fulltext
Department of Neurosurgery, the Second Hospital of Lanzhou University, Lanzhou, 730030
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Abstract:
      Objective: To explore the effectiveness of erythropoietin(EPO) administration on blood-brain barrier (BBB), brain edema, brain injury volume and NSE levels in serum after TBI in rats. Method: Experimental TBI was induced in rats by a weight-drop model. A total of 72 adult Wistar rats were divided into sham operation group(n=24), control group(n=24), EPO treatment group(n=24). Forty-eight hours after injury, extent of BBB breakdown, brain water content and injury volume were observed and 24 hours after injury neuron-specific enolase(NSE) in serum was measured. Result: BBB breakdown was lower in EPO group(67.3±13.1μg/g) than that in control group(182.8±15.9 μg/g, P<0.01). Brain edema reduced in EPO group (80.6%±0.2%) compared with that in control group(91.8%±0.6%, P<0.01). EPO treatment (17.9±4.0mm3) reduced brain injury volume compared with that in control group(37.7 ±3.8mm3, P<0.01).NSE levels in serum was lower in EPO group(6.28±3.37ng/ml) than that in control group(10.02±1.50ng/ml,P<0.05). Conclusion:Administration of EPO can keep the integrity of BBB, and reduce brain injury volume, brain edema and neuron damage.
Keywords:erythropoietin  traumatic brain injury  blood-brain barrier  brain edema  neuron-specific enolase
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