神经行为学检测和改良BBB运动功能评定在脑瘫大鼠运动功能评定中的应用

关键词：早期干预  宫内感染  脑性瘫痪  运动功能评定  Basso-Beattie-Bresnahan运动功能评定

Keywords:early intervention  intrauterine infection  cerebral palsy  motor function assessment  Basso, Beattie , Bresnahan functional scale

摘要：

      目的：探讨脑瘫大鼠运动功能评价的最佳方法。方法：采用孕鼠腹腔注射LPS制备脑瘫动物模型，随机选取对照组（A组）足月仔鼠60只， LPS组足月仔鼠120只分为早期干预组（B1组）和非干预组（B2组）各60只。各组于生后25d进行神经行为学检测，B1组中检测出的CP鼠（B1CP组）继续早期干预，B2组中检测出的CP鼠（B2CP组）常规饲养，A组中随机选取10只鼠作为对照组（A′组）； A′组、B1CP 组和B2CP组仔鼠分别于25d及42d进行神经行为学检测和运动功能评定。结果：①25d B1组鉴定出7只CP鼠，B2组13只CP鼠, A组中无CP鼠。②B1CP组第25天与第42天各项检测结果比较：悬吊试验、斜坡试验、旷场实验、拒俘反应及改良的BBB运动功能评分均有显著性差异（P﹤0.01）；B2CP组和A′组大鼠25d与42d各项检测结果比较无显著性差异（P﹥0.05）。结论：①神经行为学检测可有效鉴定CP大鼠的运动功能。②改良BBB运动功能评定适用于CP大鼠的运动功能评定。③联合应用神经行为学检测与改良BBB运动功能评定可全面反映CP大鼠运动功能状况。

Abstract:

      Objective: To explore the best methods to evaluate the motor function of cerebral palsy rats. Method: Intraperitoneal injection of LPS was used in pregnant rats to make cerebral palsy model. Sixty full-term neonatal rats were selected as control group(Group A); 120 rats from LPS group were divided randomly and averagely into a intervention group (Group B1) and a non-intervention group (Group B2) . 25th days after birth，neuroethology examination was taken in each group to identify CP rats. The CP rats in Group B1(Group B1CP) continuously received early intervention, while these ones in Group B2(Group B2CP) were fed normally; and 10 rats were randomly selected from Group A as control group (Group A′). Neuroethology examination and motor function assessment were taken in Group A′, Group B1CP and Group B2CP respectively at 25th days and 42nd days after birth. Result: ① 7 CP rats were identified in Group B1, 13 ones in Group B2, and none in Group A on 25th d. ② The seores of hanging test, slopes test, open-field experiments, resist capture reaction and improved BBB motor function assessment showed significant difference(P<0.01) in Group B1CP between 25th d and 42nd d. There was no significant difference(P﹥0.05) in Group B2CP and Group A′. Conclusion: ①Neuroethology examination could evaluate the motor function of CP rats. ②Improved BBB motor function assessment could be used to evaluate motor function in CP rats. ③combing application of neuroethology examination and improved BBB motor function assessment could reveal the everall motor function of CP rats.