针刀疗法对第三腰椎横突综合征模型大鼠成纤维细胞增殖与凋亡的影响

关键词：针刀疗法  第三腰椎横突综合征  成纤维细胞  增殖  凋亡

Keywords:acupotomy therapy  the third lumbar vertebrae transverses processus syndrome  fibroblast  proliferation  apoptosis

摘要：

      目的：观察针刀疗法对L3横突综合征模型大鼠软组织中成纤维细胞增殖与凋亡的影响。方法：建立L3横突综合征大鼠模型。68只SD大鼠随机分为针刀组、封闭组、模型组、正常对照组，每组17只大鼠。第15天对相应组别进行针刀和封闭干预。于第21、30天时，每组各处死8只大鼠，取材后，行HE染色，及免疫组化和凋亡的检测，并计算成纤维细胞的增殖和凋亡指数。结果：第21天和第30天时，针刀干预组的增殖指数、凋亡指数与模型对照组相比较有非常显著的差异（P<0.01）；针刀干预组与封闭对照组在第21天时无显著差异（P＞0.05），在第30天时有显著差异（P<0.05）。结论：针刀干预对L3横突综合征模型大鼠的组织修复有效，可能是通过调节成纤维细胞增殖与凋亡的平衡，从而防止了组织瘢痕挛缩的形成，印证了中医微观阴阳平衡理论。

Abstract:

      Objective:To observe the effect of acupotomy intervention on the proliferation and apoptosis of fibroblasts in rats with the third lumbar vertebrae transverse process syndrome. Method: Rat model of the third lumbar vertebrae transverse process syndrome was established. Sixty-eight SD rats were randomly divided into 4 groups: acupotomy group, blockade group, model group and normal control group, 17 rats in each group. On the 15th day, acupotomy and blockade were applied to the corresponding group respectively. On the 21st d and 30th d, 8 rats were sacrificed in each group. After HE staining morphological changes of muscle, tendon, and other tissues were observed. Immunohistochemistry method was used to detect the proliferation of fibroblasts and the proliferation index were calculated. TUNEL technique was used to detect the apoptosis of fibroblasts, and the apoptosis index were calculated. Result: On the 21st d and 30th d, there were very significant differences of proliferation index and apoptosis index in acupotomy group and model group(P<0.01). Compared acupotomy group with blockade group, on the 21st d there was no significant difference(P>0.05), on the 30th d there were significant differences(P<0.05). Conclusion: The acupotomy therapy is effective for the tissue repairing on rat model with the third lumbar vertebrae transverse process syndrome, and the effect may be caused by regulating the balance between proliferation and apoptosis of fibroblasts to prevent the scar formation.