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徐秋玲,陈淑萍,高永辉,王俊英,谷世喆,刘俊岭.累加电针对坐骨神经痛大鼠海马及下丘脑突触素表达的影响*[J].中国康复医学杂志,2009,24(6):498~501
累加电针对坐骨神经痛大鼠海马及下丘脑突触素表达的影响*    点此下载全文
徐秋玲  陈淑萍  高永辉  王俊英  谷世喆  刘俊岭
北京中医药大学针灸学院, 北京,100029
基金项目:国家自然基金重大研究计划(No.90709031)
DOI:
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摘要:
      目的:观察电针(EA)镇痛的累积效应,及其与海马及下丘脑突触素(SYN)表达的关系。方法: Wistar雌鼠70只,随机分为正常对照组(n=10), 慢性压迫性损伤(CCI)组(n=30),去卵巢(OVX)+CCI组(n =30)。后2组又各分为不电针、EA 2d和2周组,每组10例。OVX动物去除双侧卵巢,每天颈背部皮下注射D-半乳糖,7周后水迷宫法测试动物学习记忆能力。结扎坐骨神经造成CCI慢性痛模型。电针双侧“足三里”-“阳陵泉”穴, 1次/d,不同组分别电针2d、2周。辐射热照射测定大鼠缩腿潜伏期
关键词:针刺镇痛  累积效应  海马  下丘脑  突触素
Observation on the analgesic effect of repeated electroacupuncture and its relation to the expression of synaptophysin in rat′s hippocampus and hypothalamus    Download Fulltext
College of Acu-moxibustion, Beijing University of Chinese Medicine, Beijing,100029
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Abstract:
      Objective: To observe the cumulative analgesic effect of repeated electroacupuneture(EA) and its relation to the expressions of synaptophysin (SYN) in rats′ hippocampus and hypothalamus tissues in chronic constrictive injury(CCI) and post ovariectomy(OVX) rats′. Method: A total of 70 Wistar rats were randomized into control, CCI, CCI+EA 2 days, CCI+EA 2 weeks, OVX+CCI, OVX+CCI+EA 2 days and OVX+CCI+EA 2 weeks groups, with 10 rats in each group. For rats of the latest 3 groups, subcutaneous injection of D-galactose was given once daily for 7 weeks. Morris water maze test was conducted to evaluate the rats′ memory-learning ability. Chronic pain model was established by ligating the left sciatic nerve with a piece of catgut. EA(2/15Hz,1-2 mA) was applied to bilateral “Zusanli”(ST 36)-“Yanglingquan”(GB 34), for 30min, once daily for 2d, 2 weeks respectively. Result: Compared with control group, hyperalgesia score(HAS) increased significantly after CCI. HAS in CCI+EA 2weeks group was markedly lower than those in CCI and CCI+EA 2d groups (P<0.05), suggesting a cumulative analgesic effect after EA 2 weeks. In comparison with OVX+CCI+EA 2 weeks group, HAS in OVX+CCI+EA 2 weeks group was significantly higher(P<0.05), suggesting an attenuation of the analgesic effect after OVX. SYN immunoreaction positive product integral grey values(IGV) in rats′ hippocampal CA1 area and hypothalamic PVN in CCI+EA 2 weeks group were significantly lower (upregulation of expression) than those in CCI group (P<0.05). SYN IGV of CA1 and hypothalamus in OVX+CCI+EA 2 weeks group were significantly lower than those in OVX+CCI group (P<0.05) but obviously higher than those in CCI+EA 2 weeks group(P<0.05). Conclusion: Repeated EA (2 weeks) could provide an accumulative analgesic effect in both CCI and CCI+OVX rats, which were closely related to the upregulation of SYN expression. OVX+D-galactose administration may weaken the cumulative effect of EA.
Keywords:acupuncture analgesia  cumulative effect  hippocampus  hypothalamus  synaptophysin
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