L-谷氨酰胺对大鼠次声性脑损伤的防护效应

关键词：次声  L-谷氨酰胺  脑损伤  学习记忆  S-100β蛋白

Keywords:infrasound  L-glutamine  brain injury  learning and memory  S-100β protein

摘要：

      目的：探讨L-谷氨酰胺对16Hz 130dB次声暴露下大鼠学习记忆能力、血清S-100β蛋白的影响。方法：将60只雄性SD大鼠随机分为正常对照组、次声组、次声+药物组及药物→次声组，分别将次声组、次声+药物组及药物→次声组暴露于16 Hz 130dB次声环境中，正常对照组也置于次声舱内，但不给予次声作用。次声+药物组及药物→次声组分别在次声暴露后和次声暴露前饲料中加入药物。上述各组大鼠经7d相应处理后测试其记忆功能、S-100β蛋白水平的变化情况。结果：与正常对照组比较，次声对照组记忆功能明显下降(P<0.01)，S-100β蛋白水平有明显升高 (P<0.01)；次声+药物组、药物→次声组与次声组比较，其学习记忆功能有显著性提高(P<0.05) ，S-100β蛋白水平明显降低(P<0.05)。结论：16Hz 130dB次声可引发大鼠脑损伤，使其记忆功能受损，S-100β蛋白水平升高；L-谷氨酰胺对次声性脑损伤具有防护和治疗的效应。

Abstract:

      Objective：To study the effects of L-glutamine on learning and memory abilities and the contents of S-100β protein in serum of rats exposed to 16Hz 130dB infrasound. Method：Sixty male SD rats were randomly divided into control group, infrasound group，infrasound plus drug-treatment group and drug-prevention plus infrasound group. Learning and memory abilities and the serum concentrations of S-100β protein were measured 7 d after corresponding intervention. Result: In infrasound group compared with control group, rats′ memory abilities went down significantly（P<0.01） and serum levels of S-100β protein heightened significantly（P<0.01）. Compared with those in infrasound group，in drug-treatment group and drug-prevention group rats′ memory abilities raised（P<0.05）and the serum levels of S-100?茁 protein lowered（P<0.05）. Conclusion: Rats′ learning and memory abilities could be damaged by 16Hz 130dB infrasound, which also caused S-100β protein heightening in rats′ serum, while L-glutamine could alleviate the infrasonic injury to rats′ brains .