有氧运动对老年慢性阻塞性肺病患者骨骼肌全基因组表达的影响*

关键词：基因芯片  慢性阻塞性肺病  基因表达  有氧运动  太极拳

Keywords:gene chip  chronic obstructive pulmonary disease  gene expression  aerobic exercise  traditional Chinese shadow boxing

摘要：

      目的：研究有氧运动对慢性阻塞性肺病患者骨骼肌全基因组表达的影响及其促进健康的分子机制。方法：6例COPD患者参加了有计划的太极拳训练，在训练前和训练12周后，分别对实验对象的骨骼肌进行针吸活组织采样，经处理后采用基因芯片进行全基因组表达检测。结果：本研究将全基因组表达芯片技术、BRB基因分析软件及DAVID在线搜索的生物信息学手段相结合，对基因芯片生物信息进行初步探索。筛选出差异表达基因55条（其中10条表达下调，45条表达上调）。进行生物信息学分析后，发现有氧运动使核糖体蛋白基因（包括PRL28、PRS19、SNRPF、RPS21、SNRPE）表达下调，内毒素抑制蛋白（CRI1）基因表达上调，与COPD患者炎症细胞的抑制有关。结论：PIK3R1基因表达上调可能是导致TNF-α基因表达下调的分子机制。在众多调节机制和代谢途径中，各条基因的表达变化都与PIK3R1基因表达上调有关。

Abstract:

      Objective: In order to evaluate the effect of Tai Chi Chuan(TCC, shadow boxing) on global gene expression in human skeletal muscle and study the molecular mechanism. Method: Six COPD patients participated TCC training for 12 weeks. Needle biopsies were obtained from the skeletal muscle before and after the last training. RNA extracted from the samples was hybridized to Affymetrix U133A platform and analyzed the gene expression datum. TCC training improved physical fitness overall. Result: This study combined the gene chip technology, BRB software and DAVID searching method to get the information in the global gene expression chip. The most differently expressed genes were selected in which there were 10 ones upregulated and 45 ones downregulated. According to bioinformatical analysis, Aerobic exercise can upregulate the expression of CRI1 and downregulate expression of PRL28、PRS19、SNRPF、RPS21、SNRPE. As they have the same effect to stop the inflammatory cell growing. Conclusion: Aerobic exercise upregulates the gene of PIK3R1, which has key function in all the sanatory molecular mechanisms.