设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
张立新,佟晓杰,贾 桦,李振华.超短波对大鼠周围神经缺损修复术后神经再生的影响*[J].中国康复医学杂志,2009,24(8):695~698
超短波对大鼠周围神经缺损修复术后神经再生的影响*    点此下载全文
张立新  佟晓杰  贾 桦  李振华
中国医科大学附属第一医院康复医学科,110001
基金项目:辽宁省教育厅高校科研基金资助项目(05L449)
DOI:
摘要点击次数: 2117
全文下载次数: 1857
摘要:
      目的:通过检测小剂量超短波对脱细胞同种异体神经移植物修复大鼠坐骨神经缺损后再生神经传导速度、患侧胫前肌湿重比率、患侧L4脊髓内脑源性神经营养因子(BDNF)和降钙素基因相关肽(CGRP)的表达,来观察小剂量超短波对周围神经缺损修复术后神经再生的影响。方法:将36只Wistar大鼠随机分成3组:正常组(n=4),对照组(n=16)及实验组(n=16),对照组采用脱细胞同种异体神经移植物修复大鼠坐骨神经1cm 缺损,实验组于术后第2天开始给予小剂量超短波治疗,7min/天,1次/天,直至取材,后两组分别于术后第2、4、8、12周时检测患侧L4脊髓内BDNF及CGRP蛋白表达,术后第12周时行患侧胫前肌湿重比率及再生神经电生理检测。结果:①对照组术后第2周时患侧L4脊髓内BDNF表达已开始增高,第4周时达高峰,第8周后逐渐降低,第12周时仍显著高于正常对照组,与对照组相比,实验组只有在第8周时BDNF蛋白表达明显高于对照组(P<0.05),其余各时间点差异无显著性意义。②对照组患侧L4脊髓内CGRP蛋白表达与BDNF表达趋势基本一致,实验组在术后各时间点脊髓内CGRP蛋白表达均明显高于对照组(P<0.05)。③与对照组相比,术后第12周时实验组再生神经传导速度加快,波幅升高,潜伏时缩短,胫前肌湿重比率明显增加(P<0.05)。结论:小剂量超短波可以促进大鼠周围神经缺损修复术后神经髓鞘及轴索的再生,此作用可能与小剂量超短波上调患侧脊髓内CGRP蛋白的表达,延长BDNF表达的高峰持续时间有关。
关键词:超短波  周围神经再生  脑源性神经营养因子  降钙素基因相关肽
Effects of ultrashortwave on peripheral nerve regeneration after nerve gap repairing surgery    Download Fulltext
Nanjing North Street, Heping District,Shenyang,110001
Fund Project:
Abstract:
      Objective: To observe the effects of low dose ultrashortwave on peripheral nerve regeneration by means of detecting regeneration nerve conduction velocity, tibialis anterior muscle wet weight ratio, expressions of brain derived neurotrophic factor(BDNF) and calcitonin gene-related peptide(CGRP) of L4 spinal cord after acellular nerve allografts(ANA) repairing the sciatic nerve gap in rats. Method: Thirty-six Wistar rats were divided into three groups randomly, normal group(n=4), control group(n=16), experiment group(n=16). ANAs were put on 10 mm gap of sciatic nerve in rats of control group. 24 hours after operation, low dose ultrashortwave therapy was administrated to the rats in experiment group. 7min, once a day, until the end of experiment. Expressions of BDNF and CGRP in spinal cord of the rest two groups, 2nd w, 4th w, 8th w, 12th w after operation were detected and analyzed statistically. Regenerative nerve conduction velocity, tibialis anterior muscle wet weight ratio of injured side were also detected 12 weeks after the operation. Result: ①The expression of BDNF of spinal cord at injured site in control group increased at the 2nd week and was up to the highest at the 4th week , decreased at 8th w, but higher than normal group at the 12th week. The expression of BDNF of spinal cord in experiment group at the 8th week was higher than that in control group(P<0.05). But the difference at other time was not obvious. ②The expression tendency of CGRP correlated with BDNF. The expression of CGRP of spinal cord at injured side in experiment group was higher than that in control group at different time.(P<0.05). ③Compared with control group, regenerative nerve conduction velocity in experiment group accelerated, wave amplitude advanced, latency time shortened and tibialis anterior muscle wet weight ratio increased.(P<0.05). Conclusion: Low dose ultrashortwave can promote nerve myelin and axon regeneration.
Keywords:ultrashortwave  peripheral nerve regeneration  brain derived neurotrophic factor  calcitonin gene-related peptide
查看全文  查看/发表评论

您是本站第 37829109 位访问者

版权所有:中国康复医学会
主管单位:中国科学技术协会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计
京ICP备18060696号-2

京公网安备 11010502038612号