电针对脑缺血大鼠NF-κB及TNF-?琢表达的影响*

关键词：电针；脑缺血再灌注；海马；核转录因子  肿瘤坏死因子

Keywords:electroacupuncture  cerebral ischemia reperfusion  hippocampus  nuclear factor-κB  tumor necrosis factor-α

摘要：

      目的：探讨核转录因子-?资B (NF-?资B)活性和肿瘤坏死因子（TNF-α）在脑缺血再灌注大鼠海马表达变化的规律和电针干预对其影响。方法：将SD大鼠随机分为正常组、假手术组、模型组、电针治疗组。采用改良线栓法制备局灶型脑缺血（MCAO）再灌模型，电针“大椎”、双侧“内关”穴。运用免疫组化检测法观察海马组织NF-κB-p65蛋白的表达及核转位；用放射免疫法测定各组大鼠海马组织中TNF-α含量的变化。结果：模型各组大鼠缺血侧海马CA1区NF-κB-p65蛋白表达和海马组织TNF-α含量显著增高（P＜0.01）。与模型各组大鼠相比，电针治疗组缺血侧海马组织CA1区NF-κB-p65蛋白表达和TNF-α含量均显著降低。结论：TNF-α的表达上调后可活化NF-κB，参与脑损伤后继发性炎症反应过程，电针能抑制其活化可以减轻脑缺血再灌注时的炎症反应，发挥神经保护作用。

Abstract:

      Objective:To investigate effects of acupuncture on the expression and content of nuclear factor-κB(NF-κB) and tumor necrosis factor-α(TNF-α) in hippocampus of rat brain after cerebral ischemia/reperfusion. Method: SD rats were randomly divided into normal group, sham operation group,model group, and electroacupuncture group. The rat′s middle cerebral artery was blocked to establish cerebral ischemia（MCAO）/reperfusion model with suturing MCA.“Dazhui”(BU14),“Neiguan”(PC6) of both side were treated with electroacupuncture. The expression and content of nuclear factor-κB in hippocampus were detected with immunohistochemistry and the content of TNF-α in hippocampus were detected by means of radio-immunoassay(RIA). Result:The expression of NF-κB-p65 in ischemic hippocampi CA1 and content of TNF-α in hippocampus of rats in model group were significantly higher than those in electroacupuncture group（P<0.01,<0.05）. Conclusion: NF-κB and TNF-α may play an essential role in injury-induced inflammatory response that leads to secondary insults after brain injury.Electroacupuncture may inhibit the expression of NF-κB in hippocampus of rat brain after cerebral ischemia/reperfusion, reduce the inflammatory response and block it migrating into nucleus. Electroacupuncture can provide the protective effects on neuronal cells after cerebral ischemia/reperfusion in rats.