姜雪梅,原 林,张学全,杨 春,黄 泳,戴景兴,吴金鹏,余 磊.针刺对大鼠局部筋膜和脊髓细胞外信号调节凝酶1/2和P38丝裂原活化蛋白激酶信号通路的影响[J].中国康复医学杂志,2009,24(11):973~976 |
针刺对大鼠局部筋膜和脊髓细胞外信号调节凝酶1/2和P38丝裂原活化蛋白激酶信号通路的影响 点此下载全文 |
姜雪梅 原 林 张学全 杨 春 黄 泳 戴景兴 吴金鹏 余 磊 |
南方医科大学基础医学院解剖教研室, 广东省组织构建与检测重点实验室,广州,510515 |
基金项目:国家重点基础研究计划(973计划)项目(2007CB512705) |
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目的:观察针刺捻转拉伸大鼠皮下筋膜对局部筋膜和脊髓背角细胞外信号调节激酶1/2(extracellular signal-regulated kinase, ERK1/2)和丝裂原活化蛋白激酶P38(P38 MAPK)信号通路的影响及筋膜结缔组织的形态学变化。方法:20只SD大鼠通过随机分组,每组5只,针刺后三里组和针刺非穴位组进行手针捻转,拉伸刺激组进行拉伸刺激,采用免疫组化技术观察筋膜和脊髓组织中细胞信号蛋白的变化;利用相差显微镜观察拉伸刺激组局部皮下筋膜形态学变化。结果:组织学改变:拉伸刺激组皮下筋膜的纤维以拉伸点为中心呈向心性分布,单位面积内细胞密度增大,细胞骨架和胞核重构成“扁梭形”。 细胞信号蛋白变化:针刺组筋膜结缔组织ERK1/2和P38MAPK表达与对照组相比均有增加,但以非穴组增加显著;ERK1/2与P38MAPK在脊髓中的表达位置由胞质转向胞核,ERK1/2与空白对照组相比差异没有显著性意义;P38MAPK的表达有所增加。结论:针刺对局部浅筋膜的ERK1/2和P38有上调作用,但与脊髓中的信号蛋白增加幅度并不完全一致,说明针刺对机体除了神经调节外,筋膜结缔组织支架可能在微观的信号转导层面对局部细胞分化与增殖具有促进作用。 |
关键词:皮下筋膜/针刺 信号转导 细胞外信号调节激酶 丝裂酶原活化蛋白激酶 穴位/非穴 |
Effect of acupupuncture on modulation of extracellwar signal regulated kinase 1/2 and P38 mitogen-activated protein kinase signal transduction pathways in subcutaneous fascia and spinal medulla of rats Download Fulltext |
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Department of Anatomy, School of Basic Medical Sciences, Southern Medical University, Guang zhou, 510515 |
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Abstract: |
Objective: To observe the effect of acupuncture on the expressions of extracellular signal regulated kinase1/2(ERK1/2) and P38 mitogen-activated protein kinase(P38 MAPK) in subcutaneous fascia and dorsal horn of spinal cord of SD rats and the morphologic changes of fascia connective tissue. Method: Twenty SD rats were randomly divided into 4 groups, 5 rats in each group. Rats of acupuncture acupoint group and acupuncture non-acupoint group received hand acupuncture with twist manipulation. Rats of stretch stimulation group received acupuncture with stretch stimulation. The morphologic changes of subcutaneous fascia were observed by phase-contrast microscope. The ERK1/2 and P38 MAPK positive cells in subcutaneous fascia and spinal cord were detected by immunohistochemistry methods. Mean density was used to semiquantitatively analyse the staining results by imagepro-plus 6.0. Result: Morphologic changes: fibers of subcutaneous fascia were stretched centripetally to the center of tension, the mean density of cells inereased in unitarea the configuration of cytoplasm and nucleus were reconstructed as long spindles with unclear boundaries under tension. Changes of signal transduction protein: the expressions of ERK1/2 and P38 MAPK increased especially in acupuncture non-acupoint group; and the location of ERK1/2 and P38 MAPK at neurocyte of spinal cord transferred from cytoplasm to nucleus after acupuncture. The expression of ERK1/2 in neurocyte didn′t enhance, while the expression of P38 MAPK enhanced after acupuncture. Conclusion: Acupuncture could up-regulate the expressions of ERK1/2 and P38 MAPK in local subcutaneous fascia. But the expressions of ERK1/2 and P38 MAPK in spinal cord were not completely coincidence with the expressions in fascia. This study demonstrated that acupunture can regulate the nearoactivity. and maybe promote the cell differentiation and proliferation of local fascia, and it′s mechanism maybe due to the modulation of ERK1/2 and P38 MAPK Signal transduction pathways by destructive factors produced by mechanical stimulation of acupuncture. |
Keywords:subcutaneous fascia/acupuncture signal transduction extracellular signal regulated kinase 1/2 P38 mitogen-activated protein kinase acupoint/non-acupoint |
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