雷公藤甲素在羊膜复合异体神经移植治疗坐骨神经损伤中的应用

关键词：雷公藤甲素  移植  羊膜  周围神经  坐骨神经

Keywords:triptolide  transplantation  amnion  peripheral nerve  sciatic nerve

摘要：

      目的：探讨雷公藤甲素对羊膜复合异体神经移植促神经再生和功能恢复的作用。方法：成年SD雄性大鼠30只，随机分3组，切除10mm坐骨神经造模，造模后3组分别采用自体神经移植、异体神经移植加雷公藤药物、异体神经移植。移植术后第24周，观察移植段神经形态学、坐骨神经指数（SFI，术后第8周开始）、胫前肌湿重、单位面积移植神经中段轴突数量和髓鞘厚度。结果：移植术后第24周自体神经移植组和异体神经移植加雷公藤药物组的坐骨神经指数、单位面积移植神经中段轴突数量和髓鞘厚度、胫前肌湿重各项检测指标无显著差异（P>0.05）, 但再生神经形态和功能恢复良好，检测的各项指标明显优于异体神经移植组（P<0.05）。结论：雷公藤甲素可促进同种异体神经移植神经再生和功能恢复治疗坐骨神经损伤。

Abstract:

      Objective：To investigate the effect of triptolide on nerve regeneration and function recovery in the allogenic nerve graft bridged by amnion. Method: The sciatic nerve deficit model was performed by excising 10mm of the nerve. Thirty adult male SD rats were randomly divided into autologous nerve graft group, experimental group (allogenic nerve graft bridged by amnion and triptolide) and allogeneic nerve graft group，10 mice in each group. Twenty-four weeks after transplantation, the morphological changes，sciatic nerve function index（SFI），wet weight of bilateral tibialis anterior muscle，the number of transplanted nerve axons in unit area and the thickness of the myelin nerve axons were observed. Result: The recovery on morphology and function of regenerative nerve in autologous nerve graft group and experimental group were better than those in allogeneic nerve graft group. Among three groups there were significant differences of SFI, wet weight of bilateral tibialis anterior muscle, the number of transplanted nerve axons in unit area and the thickness of the myelin nerve axons（P<0.05）. Conclusion: Triptolide can promote nerve regeneration and function recovery in the allogenic nerve graft bridged by amnion to treat sciatic nerve deficit.