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闻万顺,廖维靖,叶祥明,杨万同,毕 博,魏 妮,张国栋,江 城.脑缺血再灌注大鼠不同时刻乳酸和磷酸肌酸/肌酸的波谱变化[J].中国康复医学杂志,2010,25(2):119~122
脑缺血再灌注大鼠不同时刻乳酸和磷酸肌酸/肌酸的波谱变化    点此下载全文
闻万顺  廖维靖  叶祥明  杨万同  毕 博  魏 妮  张国栋  江 城
武汉大学中南医院康复医学科,武汉大学脑血管病研究中心, 湖北武汉,430071
基金项目:国家自然科学基金资助项目(30370357)
DOI:
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摘要:
      摘要 目的:研究大鼠脑缺血再灌注后不同时刻乳酸(Lac)和磷酸肌酸/肌酸(PCr/Cr)的波谱变化规律。 方法:雄性Wistar大鼠32只,体重160—200g,随机分为正常对照组和实验组。实验组包括大脑中动脉阻塞 (MCAO)模型组、假手术组、单纯梗死组,每组8只。采用1H质子波谱分析,各组均作磁共振波谱(MRS)的连续观测,观察Lac和PCr/Cr波峰的改变,以积分面积表示峰域并进行比较。 结果:与正常对照组和假手术组比较,MCAO模型组缺血后出现Lac,再灌注后短暂下降继而呈上升趋势;单纯梗死组缺血出现Lac,一段时间维持后再次呈上升趋势。MCAO模型组缺血后PCr/Cr显著下降,再灌注后再次显著下降,低水平维持;单纯梗死组缺血后PCr/Cr显著下降,并在此低水平一直维持。 结论:脑缺血再灌注大鼠不同时刻Lac和PCr/Cr的波谱变化可较好地反映缺血后脑组织的能量代谢变化,有无再灌注均不能改变能量缺失变化。 关键词 脑缺血再灌注;乳酸;磷酸肌酸/肌酸;磁共振波谱 中图分类号:R493,R741 文献标识码:A 文章编号:1001-1242(2010)-02-0119-04
关键词:脑缺血再灌注  乳酸  磷酸肌酸/肌酸  磁共振波谱
The variation of magnetic resonance spectrum of lactate and phosphocreatine/creatine after cerebral ischemia/reperfusion in rats at different times    Download Fulltext
Dept. of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University,430071
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Abstract:
      Abstract Objective: To study the regulation of magnetic resonance spectrum(MRS) of lactate(Lac) and phosphocreatine/creatine(PCr/Cr) after cerebral ischemia/reperfusion in rats at different times. Method: Thirty-two male Wistar rats, weighting 160—200g, were randomly divided into normal control group and operation group, operation group included middle cerebral artery obstruction(MCAO) group, sham-operation group, and simple-infarction group, 8 rats in each every group. Proton magnetic resonance spectroscopy was used to analysis areas under the waves of Lac and PCr/Cr and compared the data of integral areas under the waves. All the groups were under continuous observation of MRS. Result: Compared with normal control group and sham-operation group, in MCAO group the wave of Lac appeared after ischemia, descended temporarily after reperfusion and then kept the trend of rising; in simple-infarction group the wave of Lac appeared after ischemia, maintain the level at sometime and then changed the trend of rising. In MCAO group the wave of PCr/Cr descended significantly after ischemia, then descended significantly again after reperfusion, maintain the low level. In simple-infarction group the wave of PCr/Cr descended significantly after ischemia, and maintain the low level all the time. Conclusion: The variation of MRS of Lac and PCr/Cr after cerebral ischemia/reperfusion in rats can reflect the energy metabolitsm in brain tissue well. Whether reperfusion or not can not change the deficiency of energy.
Keywords:cerebral ischemia/reperfusion  lactate  phosphocreatine/creatine  magnetic resonance spectrum
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