艾灸对快速老化模型小鼠海马神经干细胞分化的影响

关键词：艾灸  快速老化型小鼠  神经干细胞  海马  分化

Keywords:moxibustion  senescence-accelerated mouse prone 8  neural stem cell  hippocampus  differentiation

摘要：

      摘要 目的：研究艾灸疗法对快速老化模型（SAMP8）小鼠海马神经干细胞（NSCs）分化的影响。 方法：24只SAMP8小鼠随机分为模型组与艾灸组，12只抗快速老化型（SAMR1）小鼠作为正常对照组，艾灸组选用“百会”穴进行艾灸治疗。每天治疗1次，7d为1个疗程，共治疗3个疗程。处死前1周开始给予小鼠50mg/kg的溴脱氧尿嘧啶核苷（BrdU）腹腔注射，治疗结束后，取海马组织，用免疫荧光双标方法检测NSCs分化。 结果：①各组小鼠海马均有新生神经元免疫荧光双标阳性细胞表达。与模型组比较，艾灸组有促进或诱导NSCs向成熟神经元及未成熟神经元分化的倾向（P>0.05）。②模型组成熟星形胶质细胞神经胶质纤维酸性蛋白表达增多，艾灸能使其表达下降（P>0.05）；模型组未成熟星形胶质细胞表达减少，艾灸能促进其表达（P<0.05）。③与对照组比较，模型组少突胶质细胞增多（P<0.05）；艾灸能减少其表达（P<0.05）。 结论：经过3个疗程的艾灸治疗，能抑制小鼠海马NSCs向少突胶质细胞分化，促进其向未成熟星形胶质分化，同时其也有向神经元分化倾向。

Abstract:

      Abstract Objective：To investigate the influence of moxibustion therapy on hippocampal neural stem cells(NFCs) differentiation of senescence-accelerated mouse prone 8(SAMP8) mice. Method：Twenty-four SAMP8 mice were randomized equally into model group and moxibustion treatment group, with 12 senescence-accelerated mouse/resistance(SAMR1) mice as control group. Moxibustion on Baihui（Du20） was administered once daily for 21 consecutive days in mice in moxibustion group.After i.p bromodeoxyuridine(BrdU) 50mg/kg, mice were sacrificed 1 week later, then the proliferation and differentiation of NSCs in hippocampus were detected by immunohistochemistry，double-labeled immunofluorescent. Result：① New neurons appeared in both SAMP8 and SAMR1 groups. Compared to SAMP8 group， the expression of BrdU/MAP-2(microtube associated protein-2) and BrdU/β-tubulin Ⅲin moxibustion group had no obvious change and only showed increasing tendency (P>0.05). ②Compared to SAMR1 group, the expression of BrdU/GFAP(glialfibrillary acidic protein) increased in SAMP8 group, and decreased in moxibustion group(P>0.05). The positive cells of BrdU/ S-100 β decreased in SAMP8 group, and increased in moxibustion group(P<0.05). ③Compared to SAMR1 group，the positive cells of BrdU/GalC increased significantly（P<0.05） in SAMP8 group; after treatment of moxibustion, the expression decreased（P<0.05）. Conclusion： After moxibustion treatment for 21d, it can inhibit the hippocampal NSCs′ differentiating into oligodendrocyte and promote differentiating into immaturity astrocytes, but it also has the tendency of differentiating into neurons.