电针结合重复经颅磁刺激对局灶性脑缺血大鼠蛋白激酶表达的影响

关键词：脑缺血  电针  重复经颅磁刺激  蛋白激酶A

Keywords:cerebral ischemia  electro-acupuncture  repetitive transcranial magnetic stimulation  protein kinase A

摘要：

      摘要 目的:探讨电针(EA)结合重复经颅磁刺激(rTMS)对局灶性脑缺血大鼠蛋白激酶A(PKA)表达的影响及其治疗缺血性脑损伤的机制。 方法:Wistar大鼠75只,采用线栓法制备大鼠大脑中动脉闭塞模型,随机分为正常组、模型组、EA组、rTMS组和EA+rTMS组,通过Western印迹检测脑缺血后第7天、第14天与第28天三个不同时间点大鼠海马胞核内PKA表达的变化,并观测其神经功能评分。 结果:脑缺血后不同时间点缺血侧海马PKA灰度值,模型组在第7天时高于正常组,第28天时低于正常组（P<0.05）,第14天时与正常组相比P>0.05；EA组、rTMS组和EA+rTMS组3个时相均高于模型组,第7天、第14天时高于正常组,差异具有显著性意义(P<0.05), 第28天时与正常组相比P>0.05，其中,EA+rTMS组第7天、第14天时高于EA组、rTMS组P<0.05,EA组和rTMS组各时间点均无显著性差异(P>0.05)。EA组、rTMS组和EA+rTMS组各时间点神经功能评分均较模型组改善(P<0.01),尤以EA+rTMS组为明显。 结论:EA结合rTMS对脑卒中后神经功能的恢复具有显著的促进作用,PKA蛋白的表达增强可能是其治疗缺血性脑卒中的机制之一。

Abstract:

      Abstract Objective: To investigate the effects of electro-acupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on protein kinase A（PKA） expression after focal cerebral ischemia in adult rats and to explore the mechanism of EA combined with rTMS in treating ischemic brain injury. Method: The model of transient focal cerebral ischemia was made by occlusion of middle cerebral artery. Seventy-five Wistar rats were randomly divided into normal group, model group, EA group, rTMS group and EA+rTMS group. The expression of PKA in hippocampus was detected and the neurologic impairment rating was observed at the 7th, 14th and 28th d after infarction respectively. Result: The average gray density of PKA expression in hippocampus after focal cerebral ischemia in model group was higher at the 7th d, lower at the 28th d than that in normal group (P<0.05); higher in EA group, rTMS group, EA+rTMS group than that in model group at each time point and normal group at the 7th and 14th d(P<0.05), higher in EA+rTMS group than that in EA group, rTMS group at the 7th and 14th d, and there was no difference between EA group and rTMS group. The improvement of neural motor function was much better in EA group, rTMS group and EA+rTMS group compared with model group(P<0.01), and the improvement were the most obvious in EA+rTMS group. Conclusion: EA combined with rTMS can promote the functional recovery after cerebral arterial thrombosis, enhance the expression of PKA in hippocampus after focal cerebral ischemia, which might be one of the important mechanisms of EA combined with rTMS in treating ischemia brain injury.