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杨小立,吴明远,秦 潮.辣椒素受体-1、一氧化氮和降钙素基因相关肽在大鼠上颈段脊髓刺激诱导脑血流增加中的作用*[J].中国康复医学杂志,2010,25(6):491~496
辣椒素受体-1、一氧化氮和降钙素基因相关肽在大鼠上颈段脊髓刺激诱导脑血流增加中的作用*    点此下载全文
杨小立  吴明远  秦 潮
西安交通大学医学院第一附属医院康复医学中心疼痛科,710061
基金项目:陕西省科技攻关项目(2008K16-06);美国NIH科研基金(HL075524)
DOI:
摘要点击次数: 1936
全文下载次数: 2014
摘要:
      摘要 目的:探讨辣椒素受体-1(TRPV1)、一氧化氮(NO)和降钙素基因相关肽(CGRP)在上颈段脊髓刺激(cSCS)诱导脑血流(CBF)增加中的作用和相互关系。 方法:将雄性SD大鼠在全麻下行C1-2椎板切除暴露颈段脊髓,刺激电极放置在左侧C2脊髓背柱上。切开颅骨暴露左侧大脑皮质,CBF变化由激光多普勒血流仪测量。分析90%运动阈值(MT)cSCS在静脉注射辣椒素类似物树胶脂毒素(RTX)2μg/kg(n=9)、CGRP8-37(CGRP受体拮抗剂)2.5mg/kg(n=8)、L-NAME(一氧化氮合酶抑制剂)5.0mg/kg(n=7)前和20 min后同侧脑血流变化(%△CBF)及脑血管阻力变化(%△CVR)。 结果:60%和90% MT的cSCS使同侧大脑皮质血流增加(P<0.05,n=9)。静脉注射含有TRPV1纤维脱敏化的RTX (2μg/kg)后,90% MT的cSCS引起%△CBF由65.0%±9.5%减少到27.4%±7.2%(P<0.05,n=9),%△CVR由-28%±7.6%增加到-14.8%±4.2%(P<0.05,n=9);静脉注射CGRP8-37(2.5mg/kg) 使90% MT的cSCS引起增加的CBF降低(%△CBF:63.4%±10.4% vs. 22.7%±5.3%, P<0.05,n=8; %△CVR: -27.9%±5.9% vs. -14.8%±3.2%, P<0.05,n=8);静脉注射L-NAME(5.0mg/kg)使90% MT的cSCS引起增加的CBF显著下降(%△CBF: 58.1%±9.5% vs. 14.0%±2.5%, P<0.01,n=7; %△CVR: -20.4%±4.3% vs. -7.6%±0.6%, P<0.01,n=7)。 结论:TRPV1、NO和CGRP参与cSCS引起显著的脑血流增加或脑血管扩张,它可能为cSCS治疗缺血性脑血管病提供新的理论依据。
关键词:脊髓刺激  辣椒素受体-1  一氧化氮  降钙素基因相关肽  血管扩张
Role of TRPV1, NO and CGRP in augmentation of cerebral blood flow by upper cervical spinal cord stimulation/    Download Fulltext
Department of Pain Management, Medical Center of Rehabilitation, First Affiliated Hospital, Medical School of Xi′an Jiaotong University, Xi′an, Shaanxi , 710061
Fund Project:
Abstract:
      Abstract Objective: To explore the role and relationship of transient receptor potential vanilloid type 1 (TRPV1), nitric oxide (NO) and calcitonin gene-related peptide (CGRP) in augmentation of cerebral blood flow (CBF) by cervical spinal cord stimulation (cSCS). Method: Laminectomy was performed to expose the dorsal surface of C1-C2 spinal cord under general anesthetized male rats. The stimulus electrode was placed on the left dorsal column at cervical spinal cord (C2). Parietal craniotomy was performed to expose the left cortex for placement of laser Doppler flowmetry(LDF) probe to measure CBF. Effects of cSCS at 90% of motor threshold(MT) on ipsilateral CBF and cerebral vascular resistance(CVR) were compared before and 20min after intravenous resiniferatoxin(RTX) (2μg/kg,n=9), CGRP8-37 (2.5mg/kg,n=8)、L-NAME (5.0mg/kg,n=7). Result: The cSCS at 60% and 90% of MT applied to the dorsal column of upper cervical (C1-C2) ipsilateral spinal segments produced vasodilation in ipsilateral cerebral cortex (P<0.05,n=9). Further, only cSCS at 90% of MT was employed in following procedures. After desensitization of TRPV1-containing neural fibers with intravenous RTX(2μg/kg), cSCS-induced augmentation of CBF decreased from 65.0±9.5% to 27.4±7.2% (P<0.05, n=9). Accordingly, cerebral vascular resistance (%△CVR) increased from -28.0±7.6% to -14.8±4.2% (P<0.05, n=9). Intravenous CGRP8-37(2.5mg/kg, n=8) significantly decreased cSCS-induced %△CBF (63.4±10.4% vs. 22.7±5.3%, P<0.05) and increased %△CVR (-27.9±5.9% vs. -14.8±3.2%, P<0.05). Intravenous L-NAME(5.0mg/kg, n=7)decreased cSCS-induced %△CBF from 58.1±9.5% to 14.0±2.5%(P<0.01) and increased %△CVR from -20.4±4.3% to -7.6±0.6% (P<0.01). Conclusion: TRPV1, NO and CGRP were involved in cSCS-induced augmentation of cerebral blood flow or cerebrovasodilatation. It might provide new theories and evidences for cSCS treatment of ischemic cerebral vascular diseases.
Keywords:spinal cord stimulation  transient receptor potential vanilloid type 1  nitric oxide  calcitonin gene-related peptide  vasodilation.
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