低氧及跑台运动对大鼠内皮素和降钙素基因相关肽的影响

关键词：低氧  运动  内皮素  降钙素基因相关肽  大鼠

Keywords:hypoxia  exercises  endothelin  calcitonin gene-related peptide  rat

摘要：

      摘要 目的:观察低压低氧和跑台训练对大鼠血浆内皮素和降钙素基因相关肽的影响，为高原低氧训练适应的习服提供实验数据。 方法:40只雄性SD大鼠随机分为常氧对照组、常氧运动组、低氧对照组、低氧运动组。用MPA-心功能分析系统记录血压和心率，用放射免疫法测血浆和心肌匀浆中内皮素和降钙素基因相关肽。 结果:①与常氧对照组比较，常氧运动组体重减少（P<0.05），血浆中的内皮素（ET）降低（P<0.05），血浆中和心肌匀浆中降钙素基因相关肽（CGRP）升高（P<0.001，P<0.01）；②与低氧对照组比较，低氧运动组体重减少（P<0.01），血浆中的ET降低（P<0.01），CGRP的差异不显著；③与常氧运动组比较，低氧运动组心肌匀浆中的ET降低（P<0.05）,血浆中的CGRP也降低（P<0.001）。 结论:运动使血浆中的ET降低，CGRP升高；低氧使血浆中的ET升高，CGRP降低；低氧和运动双重刺激使心肌匀浆中的ET和血浆中的CGRP降低。

Abstract:

      Abstract Objective: To study the influence of hypobaric hypoxia and treadmill running on endothelin (ET) and calcitonin gene-related peptide(CGRP) in rats, and provide experimental data for acclimatizing to plateau hypoxia training. Method: Forty male Sprague Dawley (SD) rats were randomly divided into normoxia control group, normoxia training group, hypoxia control group, and hypoxia training group. Blood pressure and heart rate were recorded with MPA-heart function analytical system. ET and CGRP in plasma and myocardium homogenate were measured by radioimmunoassay. Result: ① Compared with normoxia control group, body weight in normoxia training group decreased（P<0.05），ET in plasma decreased（P<0.05），while CGRP in plasma and myocardium homogenate increased（P<0.001，P<0.01）；② Compared with hypoxia control group, body weight and ET in hypoxia training group reduced（P<0.01）, change of CGRP was not significant. ③ Compared with normoxia training group, in hypoxia training group ET in myocardium homogenate(P<0.05) and CGRP in plasma(P<0.001) reduced. Conclusion: Training can induce ET in plasma decreasing and CGRP increasing. Hypoxia can cause ET increasing and CGRP decreasing. Double stimulation of both training and hypoxia result in decrease of ET in myocardium homogenate and CGRP in plasma.