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曹 颖,肖 雁,吴昌学,官志忠.β淀粉样肽及冈田酸对大鼠脑内突触素和发动蛋白Ⅰ表达的影响[J].中国康复医学杂志,2010,25(9):828~832
β淀粉样肽及冈田酸对大鼠脑内突触素和发动蛋白Ⅰ表达的影响    点此下载全文
曹 颖  肖 雁  吴昌学  官志忠
贵阳医学院病理学教研室,贵阳,550004
基金项目:国家自然科学基金资助项目(30870986)、科技部国际合作项目(2006DFA33530)、贵州省科技厅基金项目黔科合J字[2007]2087号
DOI:
摘要点击次数: 2061
全文下载次数: 1934
摘要:
      摘要 目的:探讨β淀粉样肽(Aβ)及冈田酸(OA)对大鼠学习记忆能力及脑内突触素和发动蛋白Ⅰ的表达影响。 方法:采用立体定向方法将Aβ1-42及OA分别注入SD大鼠双侧海马CA1区,2个月后水迷宫实验检测大鼠学习记忆能力,Western印迹及实时聚合酶链反应(real-time PCR)检测大鼠脑组织中突触素和发动蛋白及mRNA表达水平。 结果:与对照组相比,Aβ注射组、OA注射组及Aβ+OA共同注射组均能引起大鼠学习记忆能力降低,各组脑组织中突触素、发动蛋白Ⅰ及mRNA表达均降低(P<0.01),其中以Aβ+OA共同注射组改变尤为明显。 结论:Aβ和OA均可降低大鼠学习记忆能力,降低突触素和发动蛋白Ⅰ的表达,该改变可能与AD发病机制中学习记忆能力减退有关。
关键词:β-淀粉样肽  冈田酸  突触素  发动蛋白Ⅰ  阿尔茨海默病
Influence of beta-amyloid peptide and Okadaic acid on expressions of synaptophysin and dynamin Ⅰ in rat brain    Download Fulltext
Department of Pathology, Guiyang Medical College, Guiyang,550004
Fund Project:
Abstract:
      Abstract Objective: To investigate the influence of beta-amyloid peptide(Aβ) and Okadaic acid(OA) on the learning and memory abilities of rats and the expressions of synaptophysin and dynamin Ⅰ in rat brains. Method:Aβ1-42 or OA was injected into the bilateral hippocampus CA1 of SD rats, and learning and memory abilities were evaluated at two months after injection; the protein and mRNA levels of synaptophysin and dynamin Ⅰ were surveyed by Western blotting and real-time PCR, respectively. Result: Significant reduction of synaptophysin and dynamin Ⅰ at protein or mRNA level, and impaired learning and spatial memory were observed in rats after injections of Aβ, OA and Aβ+OA as compared with controls (P<0.01), in which the group of Aβ+OA showed more obvious changes. Conclusion:Aβ1-42 and OA could decrease the learning and memory abilities of rats and inhibit the expressions of synaptophysin and dynamin Ⅰ in the rat brains, which might be a mechanism in connection with cognitive deficit of AD.
Keywords:β-amyloid peptide  Okadaic acid  synaptophysin  dynamin Ⅰ  Alzheimer′s disease
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