行为学训练对海马梗死大鼠齿状回区神经干细胞增殖能力的影响

关键词：大鼠  齿状回  巢蛋白  水迷宫  神经干细胞  增殖

Keywords:rat  dentate gyrus  nestin  water maze  neural stem cell  proliferation

摘要：

      摘要 目的：研究行为学训练对海马梗死大鼠齿状回区巢蛋白（nestin）表达的影响，探讨行为学训练对齿状回区神经干细胞增殖能力的影响。 方法：102只SD大鼠随机分为训练3d、7d、14d、21d、28d、35d、42d、49d组，制动3d、7d、14d、21d、28d、35d、42d、49d组及正常对照组。每组6只，采用光化学法造成大鼠单侧海马区梗死，造模1d后，训练组给予水迷宫训练，制动组给予制动，观察各时间点不同组大鼠梗死侧和梗死对侧海马齿状回区nestin阳性细胞的表达情况。 结果：①对照组大鼠海马齿状回区有少量nestin阳性细胞。②在梗死侧，训练第14—42天nestin的表达高于制动组（P＜0.01）。③在梗死对侧，训练第21—35天nestin的表达也高于制动组和对照组（P＜0.05）。 结论：①行为学训练可以促进单侧海马梗死大鼠齿状回区nestin阳性细胞的表达。②nestin蛋白是神经干细胞的特殊标记物，故行为学训练可以增强神经干细胞增殖能力，促进神经功能的恢复。

Abstract:

      Abstract Objective: To study the effect of behavior training on the expression of nestin in dentate gyrus (DG) in rats with left hippocampal infarction. Method: One hundred and two SD rats were randomized into 3d, 7d, 14d, 21d, 28d, 35d, 42d, 49d training groups, 3d, 7d, 14d, 21d, 28d, 35d, 42d, 49d immobilization groups and control group. Behavior training and immobilization were performed from 1d after infarction in training groups and immobilization groups respectively. Immunohistochemistry was used to detect the expressions of nestin in DG at different time points. Result: Certain expression of nestin appeared in normal DG of rats. In DG of infarction side, the expressions of nestin in 7d to 42d behavior training groups were higher than that in immobilization groups(P＜0.01). In DG of infarction opposite side, from the 21st d to 35th d the expressions of nestin in training groups were higher than that in immobilization groups and control group(P＜0.05). Conclusion: Behavior training can accelerate the expression of nestin in DG of rats with left hippocampal infarction.