温热银质针疗法三种体外留针长度银质针温度及对大鼠骨骼肌作用的比较研究

关键词：银质针  温度  留针长度  肌肉再生  波形蛋白  结蛋白

Keywords:silver needle  temperature  needle length outside  muscle regeneration  vimentin  desmin

摘要：

      摘要 目的：研究温热银质针疗法时体外留针长度与温热银质针温度及大鼠骨骼肌形态学变化的关系，以明确最适的留针长度。 方法：将54只大鼠随机分为3组采用温热银质针治疗，银针刺入鼠左后腿皮肤深度均为3cm，各组体外留针长度分别为9cm、7cm、5cm，艾条在针柄顶端点燃。使用数字测温仪测量针尖、皮肤进针点及二者中点的温度。光镜下观察针尖处肌肉组织形态学变化及波形蛋白和结蛋白表达情况。 结果：温热银质针治疗过程中，各组针尖、中点、皮肤进针点温度波动明显（体外留针9cm组针尖温度除外），第10min时最高。体外留针5cm组针尖（43.6±0.6℃）和皮肤进针点温度（46.8±1.1℃）最高，体外留针9cm组皮肤进针点温度（36.6±0.6℃）最低，体外留针9cm组针尖温度（35.2±0.7℃）与体外留针7cm组（35.6±0.7℃）无显著差异。各组大鼠骨骼肌细胞呈现损伤－修复过程，体外留针5cm组组织损伤范围大，程度严重，以瘢痕修复为主，波形蛋白和结蛋白表达少，另两组以肌细胞再生修复为主。 结论：根据温热银质针体内针身温度和大鼠骨骼肌损伤－修复情况，体外留针9cm和7cm时更合适。

Abstract:

      Abstract Objective: To determine the optimal needle length out of body during thermo-silver needle therapy, by comparing the relations between different needle lengths out of body and needle temperatures, and investigating the morphologic changes of rats′ muscle at different needle lengths out of body. Method: Fifty-four rats were randomly divided into three groups according to the needle length out of body. The silver needles were punctured into skin and muscle of rats′ left hind legs, the needle lengths out of body were 9cm, 7cm and 5cm respectively, the needle lengths staying in body were all 3cm and cones were ignited on the handles of needles. Temperature of needle tips, the puncture spots on skin and midpoints of needles were measured with semi-conductor thermistor digital thermometer. Morphologic changes of muscle and expressions of vimentin and desmin were observed under light microscope. Result: During thermo-silver needle therapy temperatures of three points of each needle in all groups fluctuated significantly and ascended at the 10th min except the needle tips in 9cm group. Among three groups, average temperature of the needle tip(43.6±0.6℃) and the puncture spot on skin(46.8±1.1℃) in 5cm group were the highest; however, average temperature of the puncture spot on skin(36.6±0.6℃) in 9cm group was the lowest. Temperature of the needle tip showed no significant difference between 9cm group (35.2±0.7℃) and 7cm group(35.6±0.7℃). Muscle cells in all groups presented injury and regeneration. Muscles in 5cm group were injured most severely, with obvious scar repairing, and the expressions of vimentin and desmin were scarce. However, regenerations were obvious in the other two groups. Conclusion: During thermo-silver needle therapy, the needle lengths of 9cm and 7cm out of body were optimal based on the needle temperature and injury-repair of rats′ muscle.