高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1、基质金属蛋白酶-9表达的影响

关键词：高压氧  脑缺血再灌注  小窝蛋白-1  基质金属蛋白酶-9

Keywords:hyperbaric oxygen  cerebral ischemia-reperfusion  caveolin-1  matrix metalloproteinase-9

摘要：

      摘要 目的：研究高压氧（HBO）对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1（Cav-1）、MMP-9表达的影响。 方法：雄性Wistar大鼠，随机分成假手术组、脑缺血再灌注组(IR)、IR+HBO、HBO组。复制局灶性脑缺血再灌注模型。HBO和IR+HBO组经0.25MPaHBO治疗5次。采用比色法、免疫组化法及Western 印迹法检测血脑屏障的通透性和Cav-1、基质金属蛋白酶-9（MMP-9）蛋白的表达。 结果：IR组第4、12、24、48、72小时脑组织伊文思蓝(EB)的含量与第0小时组相比明显增加，再灌注后第4小时EB的含量最高。IR+HBO组脑组织EB的含量明显低于IR组。IR组第4、12、24、48、72小时 Cav-1、MMP-9蛋白表达明显高于0小时组。IR+HBO组与IR组相比脑组织Cav-1、MMP-9蛋白表达显著减低。 结论：HBO降低了脑缺血再灌注时增高的脑微血管内皮细胞Cav-1蛋白和MMP-9的表达。这可能是HBO对脑缺血再灌注时血脑屏障通透性起保护作用的机制之一。

Abstract:

      Abstract Objective: To investigate the effect of hyperbaric oxygen (HBO) on caveolin-1(Cav-1) and matrix metalloproteinase-9(MMP-9) in brain tissues after cerebral focal ischemia-reperfusion in rats. Method: Male Wistar rat were randomly assigned into sham, ischemia-reperfusion (IR), HBO, and IR+HBO groups. On cerebral focal ischemia-reperfusion models, 0.25MPa HBO were applied 5 times, and 2% Evens blue (EB) was injected into tail veins 1h before execution. Expressions of Cav-1 and MMP-9, contents of EB in brain tissues were determined by Western blot technique, immunohistochemistry, and spectrophotometer, respectively. Result: In IR group, EB content in brain tissues increased significantly compared with 0h, reached it's peak at the 4th h(P<0.01). EB content in IR+HBO group was significantly lower than that in IR group. While expressions of Cav-1 and MMP-9 augmented significantly by IR injury at the 4thh, 12thh, 24thh, 48thh, and 72ndh. Compared to IR group, HBO intervention reduced their corresponding expressions levels. Conclusion: HBO intervention can reverse the increase of Cav-1 and MMP-9 caused by IR injury, which maybe one of the mechanisms for the protective effect of HBO on the permeability of blood-brain barrier in IR injury