经皮电刺激对大鼠脊髓损伤后神经胶质酸性蛋白和神经生长因子表达的影响

关键词：脊髓损伤  经皮电刺激  神经胶质酸性蛋白  神经生长因子

Keywords:spinal cord injury  transcutaneous electrical stimulation  glial fibrillary acidic protein  nerve growth factor

摘要：

      摘要 目的： 探讨经皮电刺激(TES)对大鼠脊髓损伤（SCI）后神经胶质酸性蛋白（GFAP）和神经生长因子（NGF）表达的影响。 方法： 选用成年雄性SD大鼠72只，随机分为3组：正常组(A组)、TES组（B组）和模型组（C组）。A组8只，B、C组各32只。用Allen法，复制T9脊髓不完全损伤动物模型。B组损伤后给予损伤部位上2cm位置的皮肤及右下肢小腿位置的皮肤TES治疗7d。BBB评分后，运用免疫组织化学染色和蛋白质印迹来检测GFAP、NGF的表达变化。 结果： BBB评分显示，B组与C组相比，评分增加幅度大(P<0.05)，SCI各组BBB评分均明显小于A组(P<0.05) 。免疫组化和Western 印迹检测结果显示在实验观察的7d中，B组与C组相比，GFAP的表达减少，在第5天达到最低值（P<0.05）；NGF的表达一直在增加（P<0.05）。 结论：在SCI后1—7d内，TES能抑制GFAP的表达，促进内源性NGF的合成，可能创造有利于神经再生的微环境，减少胶质瘢痕的形成。

Abstract:

      Abstract Objective： To study the impact of transcutaneous electrical stimulation（TES） on the expressions of glial fibrillary acidic protein(GFAP) and nerve growth factor (NGF) after spinal cord injury(SCI) in rats. Method：Seventy-two adult male SD rats were randomly divided into 3 groups: normal group（A group, n=8）, TES group（B group,n=32） and model group（C group,n=32）. The incomplete SCI on T9 model was made by Allen's method. TES group was given electroacupuncture on the acupoint at 2cm above the injury part skin and right shank for 7 days. Immunohistochemistry and Western blotting techniques were used to detect the expressions of GFAP and NGF after BBB evaluation. Result：The BBB score in TES group increased to 21.00 (P<0.05) compared with model group, The BBB scores in each SCI group were significantly less than that in normal group (P<0.05). The result of immunohistochemistry and Western blotting within 7 days of the experiment showed in TES group compared with model group, the expression of GFAP reduced and the lowest value appeared at the 5th day (P<0.05); NGF expression kept increasing continuously(P<0.05). Conclusion：Within 7 days after SCI, TES can inhibit the expression of GFAP and stimulate the synthesis of endogenous NGF. So it may create a favorable microenvironment for nerve regeneration and reduce the formation of glial scar.