设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
薄 海,张红英,李海英,张 勇.低氧复合运动对大鼠骨骼肌解偶联蛋白3表达及线粒体功能的影响[J].中国康复医学杂志,2012,27(1):16~21
低氧复合运动对大鼠骨骼肌解偶联蛋白3表达及线粒体功能的影响    点此下载全文
薄 海  张红英  李海英  张 勇
天津武警后勤学院部队训练医学教研室,天津,300162
基金项目:国家自然科学基金项目(30270638);武警医学院科研基金(WY2009-11)
DOI:
摘要点击次数: 2504
全文下载次数: 2709
摘要:
      摘要 目的:观察单纯低氧及低氧复合运动对大鼠骨骼肌线粒体解偶联蛋白3(UCP3)表达的影响,并探讨其对线粒体能量转换和活性氧(ROS)生成的影响。 方法:30只健康雄性SD大鼠随机分为:常氧对照组(NC, n=10)、单纯低氧组(HC, n=10)和低氧复合运动训练组(HT, n=10)。低氧干预为常压低氧帐篷,模拟11.3%的氧浓度,运动干预为低氧帐篷内53% VO2max强度的跑台训练,1h/d。4周后测定线粒体呼吸功能、ATP合成酶活力、ROS生成速率、UCP3 mRNA和UCP3蛋白表达。 结果:HC与NC组比较,态3呼吸速率(ST3)、呼吸控制比(RCR)、磷氧比(ADP/O)、ATP合成酶活力、UCP3 mRNA和蛋白表达均显著降低(P<0.05—0.01),ROS生成速率升高(P<0.05)。HT与HC组比较,ST3、RCR、ADP/O、ATP合成酶活力、UCP3 mRNA和蛋白表达均显著升高(P<0.05—0.01),ROS生成速率降低(P<0.05)。 结论:低氧复合运动可上调骨骼肌线粒体UCP3表达,抑制ROS过度生成,并通过上调ATP合成酶活力,保持线粒体能量转换效率。
关键词:低氧  解偶联蛋白3  线粒体能量转换  活性氧  骨骼肌
Effect of hypoxia combined exercise training on uncoupling protein 3 expression and mitochondrial function in skeletal muscles of rats    Download Fulltext
Logistics College of Chinese People's Armed Police Force, Tianjin, 300162
Fund Project:
Abstract:
      Abstract Objective: To observe the effect of hypoxia alone and hypoxia combined with exercise training on mitochondrial energy transferring and reactive oxygen species (ROS) generation, and to identify roles of uncoupling protein 3 (UCP3) in skeletal muscles of rats. Method: A total 30 healthy male Sprague-Dawley rats were randomly divided into three groups: normoxia control group (NC, n=10), hypoxia control group (HC, n=10), and hypoxia combined exercise training group (HT, n=10). The hypoxia animals were subjected to hypoxia exposure in normobaric hypoxic tent simulating 11.3% oxygen concentration. The exercise training animals exercised on a motor-driven rodent treadmill in normobaric hypoxic tent for 1h/d, at an intensity of 53% VO2max. After 4 weeks, mitochondrial respiration parameters, ATP synthetase activity, ROS generation rate, UCP3 mRNA and UCP3 protein expression were measured. Result: Comparing with NC group, in HC group, state 3 respiratory rate(ST3), respiratory control ratio(RCR), ratio of ADD to oxygen(ADP/O), ATP synthetase activity, UCP3 mRNA and protein expression showed dramatic reduce(P<0.05—0.01). And H2O2 generation elevated significantly(P<0.05). Comparing with HC group, in HT group, ST3, RCR, ADP/O, ATP synthetase activity, UCP3 mRNA and protein expression improved significantly(P<0.05—0.01). And H2O2 generation decreased (P<0.05). Conclusion: Hypoxia combined exercise training could markedly enhance mitochondrial UCP3 expression and ATP synthetase activity, which suppressed mitochondrial ROS generation and maintained mitochondrial energy transferring efficiency.
Keywords:hypoxia  uncoupling protein 3  mitochondrial energy transferring  reactive oxygen  skeletal muscle
查看全文  查看/发表评论

您是本站第 37832055 位访问者

版权所有:中国康复医学会
主管单位:中国科学技术协会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计
京ICP备18060696号-2

京公网安备 11010502038612号