8周跑台运动对高脂膳食大鼠脂肪细胞分化和胰岛素敏感性的影响

关键词：大鼠  高脂膳食  脂肪细胞分化  胰岛素抵抗

Keywords:rat  high-fat diet  adipocyte differentiation  insulin resistence

摘要：

      摘要 目的:探讨大鼠经高脂膳食复制胰岛素抵抗模型后，运动对其的改善作用及脂肪细胞分化在其中的影响。 方法:SD纯系雄性大鼠40只，随机分为对照组（C）、高脂组（H）、高脂运动组（HE）和普通膳食运动组（E），各运动组进行8周跑台训练，对比各组的胰岛素敏感性指标和脂肪细胞分化各指标。 结果:8周高脂膳食成功复制大鼠胰岛素抵抗模型。大鼠脂肪细胞分化各指标变化：PPARγ在E组高于C组（P＜0.01），H组高于C组; C/EBPα在H组高于其他各组（P＜0.01），E组高于C组，HE组低于H组; E组脂肪细胞分化程度最高（P＜0.01）；C组增殖指数最高，与H组和E组相比具有显著性差异（P＜0.01;P＜0.05）,HE组高于H组和E组（P＜0.05）；E组脂肪细胞体积小于C组（P＜0.01），细胞数量高于C组；H组细胞体积小于C组（P＜0.01），数量也增加（P＜0.01），HE组与H组相比脂肪细胞体积较大（P＜0.01），且数量减少。脂肪细胞分化指标与胰岛素抵抗指标间具有一定的相关关系。 结论:8周跑台运动可以改善由高脂膳食喂养引起的大鼠胰岛素抵抗现象，具体机制可能与其脂肪细胞分化的变化相关。

Abstract:

      Abstract Objective: To study the effects of aerobic exercise on insulin resistence in the rats feed by high-fat-diet and discuss whether the effect acted via the adipocyte differentiation. Method:Forty male SD rats were randomly assigned to four groups: control(C), high-fat diet control(H), high-fat diet control+exercise training(HE) and exercise training(E) group.Rats in HE and E groups were given treadmill exercise training. Insulin sensitivity and adipocyte parameters were evaluated. Result:The insulin resistence rat model was duplicated successfully by high-fat-diet. The changes of adipose differentiation were as follows: PPARγ protein of E group was higher than that of C group(P<0.01); that of H group was higher than C group, that of HE group was lower than H group. C/EBPα protein of H group was higher than that of other groups(P<0.01); that of E group was higher than C group, that of HE group was lower than H group. The adipose differentiation of E group was the highest compared with C and H groups(P<0.01); the proliferation index of C group was the highest compared with H and E groups(P<0.01 and P<0.05), that of HE group was lower than H and E groups(P<0.05). In E group size of adipose cells was smaller than that of C group(P<0.01), number of cells were more than that of C group; in H group size of adipose cells was smaller than that of C group (P<0.01), and the number increased（P<0.01）; in HE group size of adipose cells was larger than that of G group(P<0.01) and the number decreased. There was correlation between adipocyte differentiation and insulin resistance. Conclusion:Treadmill exercise for 8 weeks could improve the insulin resistence induced by high-fat diet and the inner mechanism may be related to adipose differentiation.