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孟 巍,邢艳丽,刘 帅.减重步行训练结合针刺治疗对脊髓损伤大鼠运动功能及Cdh1 mRNA表达的影响[J].中国康复医学杂志,2012,27(7):635~638
减重步行训练结合针刺治疗对脊髓损伤大鼠运动功能及Cdh1 mRNA表达的影响    点此下载全文
孟 巍  邢艳丽  刘 帅
黑龙江中医药大学,哈尔滨,150040
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摘要:
      摘要 目的:探讨减重步行训练结合针刺治疗对脊髓损伤(SCI)大鼠运动功能及Cdh1 mRNA表达的影响。 方法:将150只雄性SD大鼠随机分为针刺组(A)、步行训练组(B)、针步组(C)、对照组(D),每组30只,及假手术组(E)、空白组(F),每组15只。A、B、C、D组采用切割型脊髓损伤模型法制备SCI模型;E组仅暴露脊髓。并于术后3d开始A、B、C组相应治疗,D、E不予治疗,F组不做任何处理。术后3、5、7、14、21d对大鼠后肢运动功能进行BBB评分;及提取脊髓损伤节段组织总RNA,用实时荧光定量测定PCR检测损伤区Cdh1 mRNA的表达。 结果:21d后治疗组BBB评分明显高于D组,其中C组最高,B组次之,A组最少,各组差异有显著性(P<0.01);Cdh1 mRNA的表达C组最高,与其他各组有显著差异(P<0.01);A、B组与D组相比差异有显著性(P<0.01),A、B组差异无显著性(P>0.05)。 结论:减重步行训练或针刺治疗都对SCI大鼠运动功能及损伤部Cdh1 mRNA的表达具有良性作用,二者结合疗效更为显著。
关键词:减重步行训练  大鼠  脊髓损伤  Cdh1 mRNA
Effects of body weight support treadmill training combining with acupuncture on motion function and Cdh1 mRNA expression in rats with spinal cord injury    Download Fulltext
Heilongjiang University of Chinese Medcine,Heilongjiang,150040
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Abstract:
      Abstract Objective: To explore the effects of body weight support treadmill training combining with acupuncture on motion function and Cdh1 mRNA expression in rats with spinal cord injury (SCI). Method: One hundred and fifty male SD rats were randomly divided into acupuncture group (A), body weight support treadmill training group (B), combination group (C), control group (D), 30 rats in each group, and sham operation group (E), blank group (F), 15 rats in each group. SCI models were established by cutting method in group A, B, C and D. In group E rats' spinal cords were exposed only. Treatments were started in group A, B, C 3 days after operation, no treatment in group D, E and F. BBB scores were evaluated 3,5,7,14 and 21 days after operation, total RNA of injury spinal cord segments were extracted and expression of Cdh1 mRNA was detected by RT-PCR. Result: Twenty-one days after treatment, BBB scores in group A,B and C were markedly higher than that in group D, and the descending order was C,B,A with significant differences (P<0.01). Expression of Cdh1 mRNA peaked in group C with significant differences compared with other groups (P<0.01); there were significant differences among group A,B and D (P<0.01) and there was no significant difference between group A and B (P>0.05). Conclusion: There are positive effects of both methods of body weight support treadmill training and acupuncture, on SCI rats' motion function and expression of Cdh1 mRNA and the combination of both treatments can get better effect.
Keywords:body weight support treadmill training  rat  spinal cord injury  Cdh1 mRNA
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