葛根素对运动大鼠血液流变及运动能力的影响

关键词：葛根素  力竭运动  血液流变  大鼠

Keywords:puerarin  exhausted exercise  hemorheology  rat

摘要：

      摘要 目的：分析葛根素干预对运动训练大鼠血液流变学及运动能力的影响。 方法：60只8周龄SD雄性大鼠，30只口服葛根素组和30只对照组，葛根素组再分为10只葛根素安静组，20只葛根素+训练组（10只为运动后即刻组，10只为运动后恢复24h组）；同样30只对照组也分10只安静组，20只训练组（10只为运动后即刻组，10只为运动后恢复24h组），建立力竭游泳训练模型。通过灌服葛根素干预后，观测大鼠力竭游泳时间，测试各组大鼠全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积、红细胞沉降率（血沉）。 结果：①安静时：与对照组相比，葛根素组大鼠全血黏度显著降低[(8.41±0.76)，（6.51 ±0.92），P<0.05]，红细胞刚性指数显著降低[(1.23±0.76)，（0.98±0.72），P<0.05]和红细胞聚集指数显著降低[(16.65±2.45)，（14.78±1.55），P<0.05]；②运动后即刻时：与对照训练组相比，葛根素训练组全血黏度显著下降(P<0.05)，血浆黏度显著降低[(1.75±3.3)，（1.58±0.21），P<0.05]，红细胞聚集指数显著下降[(21.37±4.78)，（19.43±5.86），P<0.05]，红细胞刚性指数显著下降[(1.58±0.90)，（1.39±0.99），P<0.05]，红细胞压积显著降低[(0.71±0.45)，（0.57±0.43），P<0.05]；③运动后恢复24h：与对照训练组相比，全血黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积显著降低(P<0.05)，血浆黏度差异无显著性差异[(1.45±0.47)，（1.52±0.26），P>0.05]④游泳至力竭时间：葛根素组游泳时间较对照组有显著延长(P

Abstract:

      Abstract： Objective：To observe the effects of intervention of puerarin on blood hemorheology and exercise performance of rats after exercise. Method：The 60 male SD rats aged 8 weeks were selected，randomly divided two big groups: puerarin supplement groups and control groups, each group had 30 rats .each big group divided three small groups: the sedentary group, the train immediately group and the train after 24 hours recovery group, each group had 10 rats. Then established the rats exhaust-swimming train model. After puerarin supplement the exhausting-swimming time was recorded, rats' exercise performances were observed and the blood viscosity, plasma viscosity, aggregability of red blood cell(ARBC), hematocrit(HCT)，erythrocyte index of rigidity(EIR)，erythrocyte sedimentation rate（ESR）were measured. Result: ①On sedentary time: Compared with control group , the whole blood viscosity of puerarin group declined significantly[(8.41±0.76),(6.51±0.92), P<0.05], the EIR declined significantly[(1.23±0.76),(0.98±0.72), P<0.05], the ARBC of puerarin group declined significantly[(16.65±2.45),（14.78±1.55），P<0.05]；②On the train immediately: Compared with control group, the blood viscosity of puerarin group declined significantly(P<0.05), the plasma viscosity declined significantly[(1.75±3.3)，（1.58±0.21），P<0.05], the ARBC of puerarin group declined significantly[(21.37±4.78)，（19.43±5.86），P<0.05]， the EIR declined significantly [(1.58±0.90),(1.39±0.99), P<0.05], the HCT of puerarin group declined significantly[(0.71±0.45),（0.57±0.43），P<0.05]; ③After 24 hours recovery: Compared with control group, the blood viscosity, ARBC, EIR, HCT of puerarin group declined significantly(P<0.05), the plasma viscosity of puerarin group had no significant difference[(1.45±0.47)，（1.52±0.26），P>0.05];④ The exhausted swimming time: The swimming time of the puerarin group was longer than the control group(P<0.05)，and the Exhaustive Delay Rate Increased to 28%. Conclusion: Puerarin could effectively prolong the exhausted swimming time and improve hemorheological indices．