康复训练结合电针对脑卒中肢体痉挛大鼠γ-氨基丁酸能中间神经元表达的影响

关键词：γ-氨基丁酸  中间神经元  脑卒中  痉挛  康复训练  电针

Keywords:γ-aminobutyric acid  interneuron  stroke  spasticity  rehabilitation training  electro-acupuncture

摘要：

      摘要 目的：观察康复训练、电针、康复训练结合电针治疗方案对脑卒中肢体痉挛大鼠神经功能、肌张力及脑干、脊髓颈、腰膨大部位γ-氨基丁酸(GABA)能中间神经元钙结合蛋白-D28K(CB)、微白蛋白(PV)表达的影响。 方法：75只SD大鼠随机分为5组，其中模型组、康复组、电针组和综合组采用线栓法建立局灶性脑缺血动物模型，空白组不做任何处理。康复组、电针组、综合组每天治疗1次，每次30min，连续治疗6d后，检测各组肌张力、神经功能缺损，以及PV、CB的表达情况。 结果：6d治疗后，与模型组比较，康复组、电针组和综合组神经功能缺损评分值均显著降低（P<0.05），以综合组最为明显，组间比较有显著性意义（P<0.05）；肌张力均有所降低（P<0.05），以综合组最为明显，组间比较有显著性意义（P<0.05）；大鼠脑干、脊髓颈膨大、腰膨大中CB和PV积分光密度值均上升，差异有显著性意义（P<0.05），以综合组增多最为明显，组间比较差异有显著性意义（P<0.05）。 结论：康复训练、电针、康复训练结合电针三种不同的治疗方案，均可改善脑卒中肢体痉挛大鼠的神经功能、肌张力，并可上调脑干、脊髓颈膨大、腰膨大中CB、PV的表达，而尤以康复训练结合电针治疗方案效果更好。

Abstract:

      Abstract Objective：To observe effects of rehabilitation training, electro-acupuncture and rehabilitation training combined with electro-acupuncture treatment on neural function，muscle tension，and expressions of calbindin-D28k (CB) and parvalbumin（PV） in interneurons with γ-aminobutyric acid（GABA） energy in brain stem, cervical and lumbar intumenssence of spinal cord of stroke rats with limb spasticity. Method: A total of 75 SD rats were randomly divided into five groups. Model group, rehabilitation training group, electro-acupuncture group and rehabilitation training combined with electro-acupuncture treatment group were established focal cerebral ischemic animal models using the suture method; blank group hadn't any treatment. Each treatment group was treated once a day. Six days later, the neural function, muscle tension and PV as well as CB expressions were detected. Result: After 6-day treatment, in rehabilitation group, electro-acupuncture group and combined group neurological deficits scores were significantly lower than that in model group(P<0.05), the combined group was most obviously, comparison between groups showed statistically significance (P<0.05). Muscle tension of each treatment group weas lower(P<0.05), in combined group that was most obviously, comparison between groups showed statistically significance (P<0.05). In brain stem, cervical and lumbar intumescence of spinal cord of rats of each treatment group, CB and PV integrated optical density rose (P<0.05), in combined group integrated optical density increased most obviously, comparison between groups showed statistically significance (P<0.05). Conclusion: Rehabilitation training,electro-acupuncture and rehabilitation training combined with electro-acupuncture all could improve nerve function, muscle tension, and raise the expressions of CB and PV in stroke rats with limb spasticity. The effects of rehabilitation training combined with electro-acupuncture was the best.