游泳运动后自发性高血压大鼠主动脉一氧化氮的变化及其对胱硫醚-γ-裂解酶/硫化氧体系的影响

关键词：高血压  运动  一氧化氮  胱硫醚-γ-裂解酶  硫化氢

Keywords:hypertension  exercise  nitric oxide  cystathionine-γ-lyase  H2S

摘要：

      摘要 目的：观察运动对自发性高血压大鼠（SHR）主动脉一氧化氮合酶（NOS）、一氧化氮（NO）、胱硫醚-γ-裂解酶（CSE）和硫化氢（H2S）的影响，探讨运动干预后SHR大鼠内源性NO的变化对主动脉CSE/H2S体系的调节作用。 方法：选用雄性SHR大鼠16只，随机分为高血压对照组（SC组）和高血压运动组（ST组），每组8只。同时选用雄性Wistar大鼠8只，为正常对照组（WC组）。ST组大鼠进行8周、每周6次、每次90min中等强度的无负重游泳运动。 结果：8周90min游泳运动后，SC组大鼠血压较实验前显著升高(P<0.01)，ST组大鼠血压较SC组大鼠血压显著下降(P<0.01)，且与实验前相比无显著性差异（P>0.05）；ST组大鼠较SC组大鼠主动脉NOS、NO、CSE和H2S水平显著升高（P<0.05）。 结论：运动可抑制SHR的血压上升，增加SHR主动脉NOS和NO的含量，内源性NO对SHR主动脉CSE/H2S体系在运动缓解血压上升中呈促进作用，这一过程参与了运动降压的调控机制。

Abstract:

      Abstract Objective: To study the effects of exercises on blood pressure and aorta nitric oxide synthase（NOS）,nitric oxide（NO）, cystathionine-γ-lyase（CSE） and hydrogen sulfide in spontaneously hypertensive rats (SHR), then to explore the regulation effects of exercise on endogenous NO and aorta CSE/H2S in SHR. Method: Sixteen male SHR were randomly divided into the SHR control group(SC) and SHR training group (ST); 8 rats in each group. Eight male Wistar rats were the normal matched control group(WC).ST group carries on 90 min moderate swimming, without load, 6 times a week. All rats feed normal feedstuff for 8 weeks. And then the rats' blood pressure（BP） were measured each week during experimental period, the contents of aorta NOS,NO, CSE and H2S were examined. Result: After 8-weeks swimming exercise, the BP in SHR training group was little higher than before, but no significant difference. However, in SHR control group it was just opposite (P<0.01); the levels of aorta NOS,NO, CSE and H2S in SHR training group were significantly elevated（P<0.05）. Conclusion: Swimming exercise can inhibit the elevation of BP， increase the levels of aorta NOS and NO in SHR, and endogenous NO can promote the aorta CSE/H2S system in the process of exercise depressing the blood pressure elevation.