耐力训练对大鼠骨骼肌比较基因组学鉴定蛋白-58，脂滴包被蛋白基因表达的影响

关键词：耐力训练  骨骼肌  比较基因组学鉴定蛋白-58 mRNA  脂滴包被蛋白mRNA

Keywords:endurance training  skeletal muscle  comparative gene identification-58 mRNA  perilipin mRNA

摘要：

      摘要 目的：研究不同强度耐力训练对大鼠骨骼肌比较基因组学鉴定蛋白-58(CGI-58)基因和脂滴包被蛋白(perilipin)基因的表达，分析其变化规律，为运动时维持机体能量的动态平衡和代谢健康提供理论依据。 方法：选用SD雄性大鼠48只，随机分为4组，每组12只，即安静对照组（N组）；低强度耐力组（L组）；中等强度耐力组（M组）；大强度耐力组（H组）。运动负荷采用Bedford设定的大鼠运动强度等级，大鼠在跑台上进行不同坡度的递增负荷耐力训练；5周耐力训练结束后，宰杀大鼠。通过逆转录聚合酶链式反应（RT-PCR）测定腓肠肌、比目鱼肌CGI-58基因和perilipin基因表达。 结果：在腓肠肌组织中，CGI-58基因的表达随着运动强度的增加出现上升的趋势，其中在H组CGI-58表达达到最大并且高于U组（P<0.01），其次是M组和L组。在比目鱼肌组织中，各组大鼠CGI-58基因的表达为：L组、M组和H组的表达都高于N组，其中在M组时表达最强并且高于U组和H组（P<0.05）。在相同训练方式下，H组腓肠肌CGI-58 mRNA表达高于比目鱼肌（P<0.01）。在腓肠肌组织中，perilipin基因的表达随着运动强度的增加出现上升的趋势，在H组表达达到最大并且高于U组和L组（P<0.01），其次是M组。在比目鱼肌组织中，L组、M组和H组的perilipin基因表达都高于N组，在M组和H组均高于N组（P<0.05）。腓肠肌和比目鱼肌在相同训练方式下，两种肌肉perilipin mRNA表达差异也不明显（P>0.05）。 结论：5周不同强度的耐力训练可以诱发大鼠骨骼肌CGI-58 基因和perilipin基因发生差异表达，表明CGI-58 基因和perilipin基因在不同强度的耐力训练下参与了不同程度的脂肪水解调控。

Abstract:

      Abstract Objective: To research the effects of endurance training with different intensity on expressions of comparative gene identification-58 (CGI-58) and perilipin in skeletal muscles of rats, and analyze the variation and provide a theoretical basis for maintaining energy homeostasis and metabolism during exercise. Method: Forty-eight SD male rats were randomly divided into four groups, each consisting of 12 rats: the quiet control group (N), low intensity endurance training group (L), moderate-intensity endurance training group (M) and high intensity endurance training group (H). According to Bedford's exercise intensity level, the rats were trained by incremental endurance load of different slopes on a treadmill. The rats were sacrificed after 5-week endurance training, and the expressions of CGI-58 mRNA and perilipin mRNA in gastrocnemius and soleus muscles were tested by reverse transcriptase polymerase chain reaction (RT-PCR). Result: In gastrocnemius muscles, the expression of CGI-58 mRNA exhibited increasing trend with the increasing intensity exercise, in H group it reached the maximum and was higher than that in U group(P<0.01), and followed by M and L groups. In soleus muscles, the expressions of CGI-58 mRNA in L, M and H groups were higher than that in N group, in M group it reached the maximum, and exhibited significant differences compared with N and H groups (P<0.05). In the same intensity training, the expression of CGI-58 mRNA in gastrocnemius muscles was higher than that in soleus muscles in H group(P<0.01). In gastrocnemius muscle, the expressions of perilipin mRNA exhibited increasing trend with the increasing intensity exercise, in H group it reached maximun and showed extremely significant differences compared with N and L groups(P<0.01), followed by the expression in M group. In soleus muscle, the expressions of perilipin mRNA in L, M and H groups were higher than that in N group, and exhibited significant differences in M and H groups(P<0.05),vs U group. In the same intensity training, the expressions of perilipin mRNA in gastrocnemius muscle and soleus muscle showed no significant difference (P>0.05). Conclusion: The 5-week endurance training of different intensity could induce different expressions of CGI-58 mRNA and perilipin mRNA in skeletal muscles of rats. It showed that both of CGI-58 mRNA and perilipin mRNA were involved in varying degrees of hydrolysis regulation in fat by different intensity endurance training.