不同频率振动应力对RAW264.7细胞体外分化的影响

关键词：振动应力  破骨细胞  骨质疏松症

Keywords:vibration strain  osteoclast  osteoporosis

摘要：

      摘要 目的：观察不同频率振动应力对RAW264.7细胞诱导分化及活性的影响。 方法：应用复合振动仪将不同频段3—10Hz、15—35Hz、35—45Hz、50—70Hz和70—90Hz振动应变分别作用于体外诱导分化的RAW264.7细胞,振动应变加载6d时，通过抗酒石酸酸性磷酸酶（TRAP）染色及鬼笔环肽染色检查破骨细胞形成情况，通过骨吸收陷窝分析比较各组破骨细胞活性的差异。 结果：不同振动频率组形成TRAP染色阳性多核细胞数量均低于对照组，骨吸收陷窝计数亦较对照组少，差异均有显著性意义（P<0.01）。 结论：不同频率振动应力均抑制RAW264.7细胞向成熟破骨细胞分化,且随着振动频率的增加抑制能力逐渐增强。

Abstract:

      Abstract Objective：To investigate the effects of different frequencies of vibration strains on differentiation potencies of AW264.7 cells in vitro. Method：RAW264.7 cells were induced and differentiated in vitro and subjected to vibration strains at different frequencies(3—10Hz,15—35Hz,35—45Hz,50—70Hz,70—90Hz) respectively. Tartrate resistant acid phosphatase(TRAP) staining and fluorescent microscope with FITC-Phalloidin were used to assess the differentiation. Bone resorption pits were detected by scanning electron microscope to assess the function. Result：The amounts of TRAP- positive multinucleated cells and bone absorptive lacunae in vibration group were lower than control group at day 6 (P<0.01). Conclusion：Different frequencies of vibration strains could inhibit differentiation potencies of RAW264.7 cells into osteoclasts(OCs).With the frequency of vibration increasing，suppression ability of vibration strengthened gradually.