不同介入时间高压氧治疗对实验性脑出血大鼠出血灶周围水肿及水通道蛋白-4表达的影响

关键词：高压氧  介入时间  脑出血  脑水肿  水通道蛋白-4

Keywords:hyperbaric oxygen  intervention time  intracerebral hemorrhage  brain edema  aquaporin-4

摘要：

      摘要 目的： 观察不同介入时间的高压氧（HBO）治疗对实验性脑出血大鼠出血（ICH）灶周围水肿及水通道蛋白-4（AQP4）表达的影响。 方法： 应用胶原酶诱导法建立大鼠脑出血模型，将185只雄性Wistar大鼠随机分为正常组（5只）、假手术组（60只）、脑出血对照组（60只）和高压氧治疗组（60只），高压氧治疗组按高压氧介入时间不同分为6h介入组、1d介入组、2d介入组和3d介入组4个亚组，每组15只，再按不同的治疗次数分为高压氧治疗后1d、3d、5d组，每个时间点各5只鼠，高压氧治疗压力2.0ATA，稳压吸氧60min，1次/d，与末次高压氧治疗结束后24h处死。所有大鼠饲养在相同环境中，正常组饲养3d后处死，脑出血对照组与假手术组大鼠不进行任何治疗，参照高压氧治疗组进行分组，并于相应时间点断头取脑，采用干湿比重法测定脑组织的水含量，免疫组化法测定AQP4的表达。 结果： 高压氧治疗组及脑出血对照组大鼠脑组织的水含量均高于正常组及假手术组（P<0.05）；高压氧治疗组较脑出血对照组大鼠脑水含量减少（P<0.05）；高压氧治疗组间比较6h介入组较其他治疗组明显减少（P<0.05）；高压氧治疗组及脑出血对照组大鼠出血灶周围AQP4的表达均高于正常组及假手术组（P<0.05）；高压氧治疗组较脑出血对照组大鼠出血灶周围AQP4表达减少（P<0.05），高压氧治疗组间比较6h介入组较其他组减少明显（P<0.05）。 结论： HBO治疗可减少出血灶周围脑组织水含量及AQP4表达，介入治疗时间以6h为最优。

Abstract:

      Abstract Objective： To study the effect of different therapeutic intervention time of hyperbaric oxygen(HBO) on perihematomal edema and expression of aquaporin-4(AQP4) in rats with intracerebral hemorrhage(ICH). Method：A total of 185 male Wistar rats were randomly divided as normal group(n=5), sham operation group（n=60）, ICH control group (n=60) and HBO therapy group(n=60). The collagenase-induced method was used to set up the intracerebral hemorrhage rat models. The HBO therapy was applied at different intervention times (6h,1d,2d or 3d in 4 intervention time subgroups， each subgroup 15 rats) after operation. The HBO therapy was administered by using 100% oxygen at 2.0 ATA in a steady pressure for 60min, once daily. Rats were sacrificed at 24h after 1,3,5 sessions of HBO therapy. The other groups hadn't any treatment. Each intervention time point had 5 rats. The water content of brain tissue was measured by dry-wet method, the expression of AQP4 was detected by immunohistochemical staining. Result：The brain water content and AQP4 level in the perihematomal area in HBO therapy group and ICH control group were higher than that in the normal group and the sham group(P<0.05). HBO therapy group significantly reduced the brain water content and AQP4 level in the perihematomal area compared with ICH control group (P<0.05), however, the effect of HBO, which applied at 6h intervention time point, was superior to that applied at 1d, 2d, and 3d intervention time points after ICH(P<0.05). Conclusion： HBO therapy can decrease the brain water content and AQP4 level in the perihematomal area. The effect of HBO, intervention 6h,was superior to that at 1d, 2d and 3d.