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徐 波,余 锋,张宪亮,季 浏.跑台运动对D-半乳糖阿尔茨海默病大鼠海马β-淀粉样前体蛋白和Tau蛋白基因表达的影响[J].中国康复医学杂志,2014,29(11):1010~1015
跑台运动对D-半乳糖阿尔茨海默病大鼠海马β-淀粉样前体蛋白和Tau蛋白基因表达的影响    点此下载全文
徐 波  余 锋  张宪亮  季 浏
华东师范大学“青少年健康评价与运动干预”教育部重点实验室,上海,200241
基金项目:国家自然科学基金面上项目(31371204)
DOI:
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摘要:
      摘要 目的:探讨8周的有氧跑台运动对D-半乳糖模型阿尔茨海默病(AD)大鼠海马β-淀粉样前体蛋白(APP)和Tau蛋白及其激酶糖原合成酶激酶-3β(GSK-3β)mRNA表达的影响。 方法:选用健康雄性8周龄SD大鼠16只,随机分为对照组(C组,n=8),运动组(T组,n=8)。两组大鼠连续8周经腹腔按120mg/kg·d的浓度注射D-半乳糖造模AD大鼠,同时对T组大鼠进行连续8周,每周5次的有氧跑台运动。运用实时荧光定量RT-PCR的方法检测两组大鼠海马APP、Tau蛋白和GSK-3β的mRNA表达水平。 结果:8周的有氧跑台运动显著下调了T组大鼠海马APP、Tau蛋白和GSK-3β的mRNA表达水平;其中,APP的下调幅度为42%(P<0.05),Tau蛋白的下调幅度为45%(P<0.01),GSK-3β的下调幅度为38%(P<0.05)。 结论:8周的有氧跑台运动有效抑制了APP和Tau蛋白的基因表达水平,其机制可能是与运动抑制了GSK-3β的基因表达水平有关。
关键词:跑台运动  阿尔茨海默病  β-淀粉样前体蛋白  Tau蛋白  糖原合成酶激酶-3β
Effects of treadmill exercise on β-amyloid precursor protein and Tau protein gene expressions in hippocampus of D-galactose Alzheimer's disease rats    Download Fulltext
Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, Shanghai, 200241
Fund Project:
Abstract:
      Abstract Objective: To explore the effects of 8-week aerobic treadmill training on expressions of β-amyloid precursor protein (APP), Tau protein and glycogen synthase kinase-3β (GSK-3β) mRNA in hippocampus of D-galactose (D-gal) Alzheimer's disease (AD) rats. Method: Sixteen male Sprague-Dawley(SD) rats were randomly divided into control group (C, n=8), exercise group(T,n=8). All rats of two groups were injected D-gal 120mg/(kg·d) in abdominal for 8 weeks to obtain AD model rats. Group T was given aerobic treadmill, 5 times a week for 8 weeks. The expression levels of APP,Tau and GSK-3β in hippocampus of all rats were inspected by real-time fluorescence quantitative RT-PCR. Result: After 8-week aerobic treadmill exercise made the expressions of APP mRNA (42%, P<0.05), Tau mRNA (45%, P<0.01) and GSK-3β (38%, P<0.05) were down-regulated significantly in hippocampus of D-gal AD rats. Conclusion: Eight-week aerobic treadmill training could made some inhibition effects on the expressions of APP and Tau mRNA in D-gal AD rats. The mechanisms might be related with the lower expression level of GSK-3β mRNA, which was down-regulated by 8 weeks aerobic treadmill exercise and would made some important regulation effects on the levels of APP and Tau protein expression.
Keywords:treadmill exercise  Alzheimer's disease  amyloid precursor protein  Tau protein  glycogen synthase kinase-3β
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