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张良清,周 慧,杨 琳,黄剑平,杨万章,李光林.目标肌肉神经分布重建大鼠模型及低频电刺激的效果研究[J].中国康复医学杂志,2015,(1):3~9
目标肌肉神经分布重建大鼠模型及低频电刺激的效果研究    点此下载全文
张良清  周 慧  杨 琳  黄剑平  杨万章  李光林
广州医科大学研究生学院,广州,510182
基金项目:国家自然科学基金重点项目(61135004);深圳市知识创新计划项目(JCYJ20130402113127532)
DOI:
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摘要:
      摘要 目的:利用大鼠模型开展目标肌肉神经分布重建(TMR)研究,使用低频电刺激,观察电刺激对神经移植后的再生和减轻骨骼肌失神经萎缩程度的效果。 方法:所有大鼠随机分为正常对照组、失神经支配组、TMR模型组及电刺激组。制作TMR大鼠模型,将大鼠右侧正中神经移植到胸大肌上,术后2天开始进行低频电刺激。利用植入的肌内电极采集大鼠双侧胸大肌的肌电信号;采用骨骼肌收缩的力学分析方法检测胸大肌的最大单收缩力及最大强直收缩力;应用肌湿重维持率检测胸大肌失神经萎缩情况。 结果:①第4周与第1周相比,TMR模型组右侧胸大肌的肌电信号轻微增加;电刺激组右侧胸大肌的肌电信号明显增强;失神经支配组几乎观察不到右侧胸大肌的肌电信号;正常对照组的肌电信号基本不变。②正常对照组的最大单收缩力及最大强直收缩力均明显大于TMR模型组和电刺激组(P<0.01),电刺激组则明显大于TMR模型组(P<0.05)。③TMR模型组和电刺激组的胸大肌湿重维持率均明显高于失神经支配组(P<0.01),电刺激组则明显高于TMR模型组(P<0.05)。 结论:使用低频电刺激TMR大鼠模型,对促进神经移植后的再生和减轻骨骼肌失神经萎缩有积极作用。
关键词:目标肌肉神经分布重建  低频电刺激  周围神经再生  肌萎缩
Effects of low frequency electrical stimulation in targeted muscle reinnervation rat model    Download Fulltext
Graduate Schools, Guangzhou Medical University, Guangzhou, 510182
Fund Project:
Abstract:
      Abstract Objective: To assess effects of low frequency electrical stimulation on nerve regeneration and denervation muscle atrophy prevention in targeted muscle reinnervation (TMR) rat model. Method: All rats were randomly divided into control group, denervation group, TMR group and electrical stimulation (ES) group. TMR rat model was created by transferring the medial nerve of rats into their pectoralis major muscle and ES treatment was carried out 2 days after operation. An implanted electrode was used to record the intramuscular myoelectric signals from the pectoralis major muscle. The maximum single contractile force and force titanic tension of denervation the pectoralis major muscle were detected by mechanics analysis of skeletal muscle. Besides, the extent of muscle atrophy was detected by the rate of the muscle wet weight preservation. Result: ①In TMR group, the intramuscular myoelectric signal on the right side of pectoralis major muscle was slightly higher in week 4 in comparison to week 1. The intramuscular myoelectric signal on the right side of pectoralis major muscle became obvious strong in week 4 in ES group. Besides, bilateral pectoralis major muscle generated strong myoelectric signals in control group, while the intramuscular myoelectric signal could not be observed in denervation group. ②The maximum single contractile force and force titanic tension in control group were higher significantly than that in TMR group and ES group (P<0.01), and ES group was higher significantly than TMR group (P<0.05). ③The rate of the muscle wet weight preservation in TMR group and ES group were significantly higher than that in denervation group (P<0.01), and ES group was higher significantly than TMR group (P<0.05). Conclusion: The TMR rat model was developed successfully, and the transferred nerve regenerated and redistributed in targeted muscle. Low frequency electrical stimulation had positive effects on nerve regeneration and denervation muscle atrophy prevention in TMR rat model.
Keywords:targeted muscle reinnervation  low frequency electrical stimulation  peripheral nerve regeneration  amyotrophy
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