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陈吉祥,林浴坤,吴羽楠,周一平,林如辉,陶 静,陈立典.电针对脑缺血再灌注大鼠学习记忆功能及海马组织Nogo-A/NgR表达的影响[J].中国康复医学杂志,2015,(3):219~223
电针对脑缺血再灌注大鼠学习记忆功能及海马组织Nogo-A/NgR表达的影响    点此下载全文
陈吉祥  林浴坤  吴羽楠  周一平  林如辉  陶 静  陈立典
福建中医药大学康复医学院,福州,350108
基金项目:福建省康复技术协同创新中心资助项目(X2012004-协同)
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摘要:
      摘要 目的:观察电针对脑缺血再灌注大鼠学习记忆功能及海马组织Nogo-A/NgR表达的影响,探讨电针治疗脑卒中后认知功能障碍的作用机制。 方法:将45只雄性SD大鼠随机分为假手术组、模型组和电针组,每组均15只。模型组和电针组参照Longa线栓法建立大鼠局灶性脑缺血再灌注模型。电针组针刺 “百会穴”和“神庭穴”,共干预7天;假手术组和模型组在同等条件下饲养和抓取。利用Morris水迷宫实验评估大鼠的学习记忆功能;TTC染色法观察脑梗死体积;免疫印迹法检测脑梗死侧海马组织Nogo-A及其受体NgR的表达。 结果:与模型组相比,电针组大鼠逃避潜伏期和找到平台所经过的路程缩短,跨越平台次数增加(P<0.05);电针组大鼠脑梗死体积减小(P<0.05);电针组大鼠海马区Nogo-A及其受体NgR的表达量降低(P<0.05)。 结论:电针可改善脑缺血再灌注大鼠的学习记忆功能,其作用机制可能与抑制脑梗死侧海马组织中Nogo-A及其受体NgR的表达有关。
关键词:电针  脑缺血  学习记忆  Nogo-A  NgR
Effects of electroacupuncture on learning and memory and the expression of Nogo-A and NgR in rats with focal cerebral ischemia-reperfusion    Download Fulltext
Fujian University of Traditional Chinese Medicine,Fuzhou,35012
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Abstract:
      Abstract Objective:To investigate the effects of electroacupuncture on learning and memory and Nogo-A/NgR expression in hippocampus of rats with focal cerebral ischemia-reperfusion. Method:Forty-five male adult SD rats were randomly divided into the sham operation control group (SC group), the ischemia model group (IC group) and the electroacupuncture group (EA group), n=15 for each group. The focal cerebral ischemia-reperfusion model was setup by suture method in IC and EA groups. The rats of EA group received electroacupuncture treatment at Shenting (DU24) and Baihui (DU20) for 7 days. Learning and memory ability was tested by Morris water maze. The cerebral infarct volume was assessed using TTC staining and Western blot was performed to evaluate the expression of Nogo-A and NgR in hippocampus of the cerebral ischemia side. Result:The latency and route distance for rats to reach the hidden platform were markedly decreased in EA group compared with that in IC group in the Morris water maze test, whereas the times that rats crossed the location of the platform were significantly increased (P<0.05 versus IC group). TTC staining showed that electroacupuncture treatment profoundly reduced cerebral infarct volumes compared with that of IC group (P< 0.05). Furthermore, the expression of Nogo-A and NgR in the hippocampus of the cerebral ischemia side was significantly downregulated in EA group (P<0.05, versus IC group). Conclusion:Learning and memory of rats with focal cerebral ischemia-reperfusion could be improved with electroacupuncture treatment. The mechanism might be based on the inhibition of the expression of Nogo-A and NgR.
Keywords:electroacupuncture  cerebral ischemia  learning and memory  Nogo-A  NgR
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