磁场对大鼠脑缺血再灌注后胶质纤维酸性蛋白表达的影响

关键词：磁场  缺血/再灌注  胶质纤维酸性蛋白

Keywords:pulsed magnetic  ischemia/reperfusion  glial fibrillary acidic protein

摘要：

      摘要 目的:探讨磁场对大鼠脑缺血再灌注后胶质纤维酸性蛋白（GFAP）表达的影响。 方法:线栓法制作大脑中动脉闭塞（MCAO）脑缺血2h再灌注模型。将24只雄性SD大鼠随机分成模型组(n=12)、磁疗组(n=12), 每组再分为3d、7d两个亚组，每组6只。磁疗组在缺血再灌注12h后予磁场处理（０—10.5mT，频率50Hz，20min/次，1次/d）。模型组干预过程中除不开机外，其余过程同磁疗组。在治疗3d、7d后(处死前)对大鼠进行神经功能缺损评分(mNSS)，脑组织切片采用免疫组织化学染色方法观察脑缺血周围GFAP表达变化。 结果：磁疗7d组大鼠mNSS评分较模型组降低(P<0.05)；磁疗组脑缺血周围GFAP免疫组织化学染色反应阳性细胞计数增加（P<0.05）。 结论：磁疗可促进大鼠脑缺血再灌注后GFAP的表达，促进脑组织修复。

Abstract:

      AbstractObjective:To explore the effect of magnetic field on the expression of glial fibrillary acidic protein (GFAP) after cerebral ischemia/reperfusion in rats. Method:The model of cerebral ischemia/reperfusion in rats was induced with intraluminal middle cerebral artery occlusion (MCAO) with nylon monofilament suture. Twenty-four male adult SD rats were randomly divided into model group (n=12) and magnetic therapy group (n=12), then each group was randomly divided into 3d and 7d subgroups, with 6 rats in each subgroup.The magnetic therapy group was treated with pulsed magnetic field(0—10.5mT, 50Hz, 20min/times, 1 times/d) after operation, the model group was treated in the same way but turning off the power. Both groups were evaluated with the modified neurological severity score (mNSS) at 3d and 7d after treatment (before being executed), and brain tissue slices were immunohistochemically stained to observe the expression of GFAP around the ischemic sites. Result：Compared to the model group, in 7d magnetic therapy subgroup the mNSS scores decreased significantly(P<0.05), and the number of GFAP increased significantly（P<0.05）. Conclusion：Pulsed magnetic field can increase the expression of GFAP after cerebral ischemia/reperfusion in rats，and promote the tissue repair.