电针百会对APP/PS1转基因小鼠学习记忆能力及Tau蛋白磷酸化的影响

关键词：电针  阿尔茨海默病  学习记忆  Tau

Keywords:electroacupuncture  Alzheimer's disease  learning and memory  Tau

摘要：

      摘要 目的：基于Tau蛋白磷酸化探讨电针百会对APP/PS1转基因痴呆模型小鼠学习记忆能力的影响及其可能机制。 方法：30只4月龄雌性APP/PS1转基因小鼠随机分为模型组、百会组和非穴组，10只同窝阴性野生小鼠为野生组；百会组电针百会穴，非穴组电针非穴，干预28d。采用Morris水迷宫实验观察小鼠学习记忆能力；尼氏染色观察小鼠海马神经元形态结构变化；蛋白免疫印记杂交技术（Western blot）检测小鼠海马组织在Ser-396位点上Tau蛋白磷酸化水平。 结果：与模型组相比，百会组可改善小鼠的学习记忆能力（P＜0.05），减轻海马神经元形态结构的损伤，降低海马组织中Ser-396位点上Tau蛋白磷酸化水平（P＜0.05），非穴组与模型组相比无显著性差异（P＞0.05）。 结论：电针百会能够改善APP/PS1转基因痴呆模型小鼠的学习记忆能力，其机制可能与抑制Tau蛋白磷酸化有关。

Abstract:

      Abstract Objective: To explore the effects of electroacupuncture at Baihui (DU20) on learning and memory ability and its possible mechanism through the phosphorylated Tau in APP/PS1 transgenic mice. Method: Thirty female APP/PS1 transgenic dementia model mice were randomly divided into model group, DU20 group and non-acupoint group, 10 wild type mice consisted wild group. The DU20 group received electroacupuncture at Baihui (DU20) and the non-acupoint group received electroacupuncture at non-acupoint for 28 days. Learning and memory ability was tested with Morris water maze. The morphology and structure of hippocampus neurons were observe by Nissl's staining. The level of Tau phosphorylation at Ser-396 point was detected by Western blot. Result: Compared with the model group, electroacupuncture at DU20 could ameliorated learning and memory ability of APP/PS1 mice (P＜0.05), decreased the injury of neurons in morphology and structure and the level of Tau phosphorylation at Ser-396 in hippocampus (P＜0.05), and there was no significant difference between model group and non-acupoint group (P＞0.05). Conclusion: Electroacupuncture at DU20 could ameliorate learning and memory ability in APP/PS1 transgenic dementia model mice, the mechanism may be related to the Tau phosphorylation in hippocampus.