正中神经电刺激对脑外伤后昏迷大鼠前额叶皮质及下丘脑Orexin-A及其受体OX1R表达变化的影响

关键词：正中神经电刺激  脑外伤  促醒  昏迷  Orexin-A  OX1R

Keywords:median nerve electrical stimulation  traumatic brain injury  wake-promoting  coma  Orexin-A  OX1R

摘要：

      摘要 目的：探讨正中神经电刺激（MNES）对脑外伤（TBI）昏迷大鼠的促醒作用及其可能机制。 方法：将SD大鼠分为3组：空白对照组、假刺激组和刺激组。应用MNES治疗脑外伤后昏迷大鼠，观察其行为学变化，并用免疫组织化学、Western-blot、ELISA技术检测各组大鼠前额叶皮质（PFC）和下丘脑组织的Orexin-A及OX1R含量。 结果：刺激组30只大鼠中21只出现翻正反射，假刺激组30只中7只出现翻正反射；将3组的Orexin-A及OX1R含量进行两两比较，发现刺激组Orexin-A及受体水平高于其他两组，差异有显著性意义（P＜0.05）。 结论：MNES可作为脑外伤后昏迷的有效促醒手段，其机制可能与PFC及下丘脑部位Orexin-A及OX1R水平上调有关。

Abstract:

      Abstract Objective： To investigate the wake-promoting action and potential mechanisms of median nerve electrical stimulation(MNES) in traumatic brain injury(TBI)-coma rats. Method： Ninety rats were divided into three groups： control, sham-stimulated (TBI) and stimulated (TBI+MNES) group. MNES was applied to rats after TBI-coma models were established. Then, behaviors of rats were observed and Orexin-A and OX1R in prefrontal cortex(PFC) and hypothalamus area were examined via immunohistochemistry, Western-blotting and ELISA techniques. Result： In stimulated group, 21 rats exhibited righting reflex and in TBI group, 7 rats had the same responses. In stimulated group, both in PFC and hypothalamus area, Orexin-A and OX1R levels were higher than those in other groups (P＜0.05). Conclusion： MNES might be an effective wake-promoting way for TBI-coma. The mechanism might be due to upregulating the excitatory hypothalamic neuropeptide Orexin-A and OX1R expression in PFC and hypothalamic area.