“醒脑开窍”针刺法对脑缺血再灌注大鼠模型早期脑内血管内皮生长因子与胶质纤维酸性蛋白表达的影响

关键词：“醒脑开窍”针刺法  脑缺血再灌注  大鼠  血管内皮生长因子  胶质纤维酸性蛋白

Keywords:"Xingnao Kaiqiao" acupuncture method  cerebral ischemia reperfusion  rat  vascular endothelial growth factor  glial fibrillary acidic protein

摘要：

      摘要 目的：探讨“醒脑开窍”针刺法对局灶性脑缺血再灌注模型大鼠早期脑内血管内皮生长因子（VEGF）与胶质纤维酸性蛋白（GFAP）表达的影响。 方法：健康成年雄性SD大鼠随机分为假手术组、模型组、电针组和电针对照组，每组20只。采用线栓法制作大鼠大脑中动脉缺血再灌注模型。电针组和电针对照组主穴取双侧内关、水沟、三阴交、百会，采取“醒脑开窍”法行电针治疗30min。首次针刺在动物造模成功后24h内进行，其后每天上午针刺1次，每7天为一疗程（针刺6d，休息1d）。假手术组、模型组常规饲养于笼内，不进行任何干预治疗。各组大鼠在模型制作成功后第7天、第14天两个时间点取10只进行Longa神经功能评估、免疫组化SP法观察VEGF与GFAP的表达。 结果：电针对照组和假手术组大鼠无神经功能缺损症状，在模型制作成功后第7天、第14天时，电针组大鼠神经功能恢复明显优于模型组。免疫组化SP法检测电针对照组和假手术组可见少量VEGF与GFAP的表达，模型组大鼠脑梗死后第7天，脑缺血周围出现VEGF与GFAP表达增多，第14天时增多明显，与假手术组比较均有明显差异。电针组VEGF与GFAP表达在各时间点较模型组增加更显著。 结论：“醒脑开窍”针刺法能通过促进脑局灶性缺血再灌注大鼠脑内VEGF与GFAP的表达，有效改善大鼠局灶性脑梗死后的神经功能恢复。

Abstract:

      Abstract Objective： To investigate effects of "Xingnao Kaiqiao" acupuncture therapy on expressions of vasular endothelial growth factor（VEGF） and glial fibrillary acidic protein（GFAP） after cerebral ischemia reperfusion in rats at early stage. Method： Healthy adult male SD rats were randomly divided into sham operation group, model group, electro-acupuncture group and electroacupuncture control group, with 20 rats in each group. The middle cerebral ischemia reperfusion model was established by suture method. Electroacupunctures were performed in the electroacupuncture group and electroacupuncture control group with "Xingnao Kaiqiao" method at main acupoints of Neiguan (PC6), Shuigou (DU26), Sanyinjiao (SP6), Baihui (DU20) of both sides, for 30min. The first session of acupuncture was administered in 24h after successful modeling. Then acupunctures were followed once daily in every morning, every 7d for a course (acupuncture 6d and rest 1d). The sham operation group, model group were conventionally fed in the cage, without any intervention therapy. Ten rats of each group were assessed with Longa's score, and the expressions of VEGF and GFAP were detected with the method of immunohistochemical SP at the 7th d and 14thd. Result： Electroacupuncture control group and sham operation group were presented no neurological deficit. In electroacupuncture group of rats' nerve functional recovery were significantly better than that in model group at the 7th d and 14thd after modeling. A small amount expressions of VEGF and GFAP were detected in the sham operation group and the electroacupuncture control group with the method of immunohistochemical SP. At the 7th d after modeling expressions of VEGF and GFAP increased around the cerebral ischemia regions at the 14th d increased significantly, compared with the sham operation group, there were significant differences. In electroacupuncture group the expressions of VEGF and GFAP at each time point increased more significantly than those in the model group. Conclusion： The "Xingnao Kaiqiao" acupuncture therapy can promote the recovery of neurological function of focal cerebral ischemia reperfusion in rats which may be associated with increase of VEGF and GFAP in peri-infarct regions.