电针对局灶性脑缺血再灌注模型大鼠学习记忆作用的氧化应激机制

关键词：脑缺血  电针  学习记忆  氧化应激  凋亡

Keywords:cerebral ischemia  electroacupuncture  learning and memory  oxidative stress  apoptosis

摘要：

      摘要 目的：通过氧化应激探讨电针神庭、百会对局灶性脑缺血再灌注大鼠学习记忆的影响，阐明其治疗脑缺血后认知功能障碍的可能机制。 方法：雄性SD大鼠72只，随机分为假手术组、模型组、电针组、非穴组，每组18只大鼠。以大脑中动脉闭塞（MCAO）法建立大鼠局灶性脑缺血再灌注模型。电针组、非穴组行电针干预7天。各组在造模后3d开始采用Morris水迷宫检测学习记忆能力，免疫组化、免疫蛋白印迹和RT-PCR检测caspase-3表达,用化学比色法检测SOD、MDA和GSH-PX。 结果：与其他组相比，电针神庭、百会穴可明显改善MCAO大鼠的神经功能缺损症状；改善MCAO大鼠学习记忆能力；降低皮质caspase-3表达；增强SOD、GSH-PX的活性，降低MDA的含量。 结论：电针通过抑制氧化应激、控制神经细胞凋亡，来实现改善局灶性脑缺血再灌注大鼠学习记忆能力，保护神经细胞的损伤。

Abstract:

      Abstract Objective: To investigate the effect of electroacupuncture (EA) ameliorating learning and memory ability in rats with focal cerebral ischemia/reperfusion（I/R） via inhibiting oxidative stress and to clarify the possible mechanism of EA in treatment of cognitive impairment after cerebral ischemia. Method: Seventy-two male adult SD rats were randomly divided into the sham operation control group (SC group), the ischemia model group (MCAO group), the electroacupuncture group (EA group) and electroacupuncture non-point group (non-point group). Each group had 18 rats. Middle cerebral artery occlusion (MCAO) was performed to establish the focal cerebral I/R injury model. The EA group and non-point group received EA for 7d. All rats were tested by Morris water maze to investigate learning and memory ability. Immunohistochemistry, Western blot and RT-PCR were used to test the expression of caspase-3, and using chemical assay to detect SOD, MDA and GSH-PX. Result: Compared with other groups, EA at Shenting (DU24) and Baihui (DU 20) acupoints alleviated neurological deficits significantly and promoted learning and memory ability; reduced caspase-3 expression; enhanced activities of SOD, GSH-PX, reduced the content of MDA. Conclusion:Electroacupuncture can ameliorate learning and memory ability of cerebral I/R injured rats and protect nerve cell damage. Its mechanism may be related to inhibiting oxidative stress and modulating the expression of apoptosis-related gene.