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董军涛,林阳阳,燕铁斌,梁慧英,吕 晓,何晓阔,眭明红.不同形式的运动训练对血管性痴呆大鼠学习记忆及海马区突触可塑性的影响[J].中国康复医学杂志,2016,(7):716~722
不同形式的运动训练对血管性痴呆大鼠学习记忆及海马区突触可塑性的影响    点此下载全文
董军涛  林阳阳  燕铁斌  梁慧英  吕 晓  何晓阔  眭明红
中山大学孙逸仙纪念医院康复医学科,广州,510120
基金项目:国家自然科学基金面上项目(81171863)
DOI:
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摘要:
      摘要 目的:观察自主运动、强迫运动和功能性电刺激诱导的运动对血管性痴呆(vascular dementia, VD)大鼠学习记忆、海马区突触可塑性的影响。 方法:成年Wistar雄性大鼠,体重250—300g;用10%水合氯醛(300mg/kg)行腹腔注射麻醉,采用双侧颈总动脉永久性结扎法制作血管性痴呆模型。造模成功后大鼠在跑轮中适应3天(剔除运动量不能达到每天270m的大鼠),采用随机数字法分为假手术组、模型组、自主运动组、强迫运动组和功能性电刺激组,每组各8只。假手术组:仅暴露双侧颈总动脉,但不接扎,术后大鼠置于笼中自由活动;模型组:采用双侧颈总动脉永久性结扎法制作VD模型,术后大鼠置于笼中自由活动;自主运动组:造模1周后大鼠在跑轮(直径31.8cm,宽度10cm,旋转阻力约相当于100g物体的重力)上自由运动,用传感器记录跑过的圈数,每天270圈;强迫运动组:造模1周后大鼠在电动跑轮(直径31.8cm,长度40cm,转速9r/min)上运动,每天治疗30min;功能性电刺激组:造模1周后开始治疗,诱导大鼠前肢产生以9m/min行走时的动作,每天治疗30min。以上五组于治疗14d后,采用新奇事物识别实验测试大鼠学习记忆能力。取大鼠海马组织采用Western blot技术检测上述各组SYN、SYP、PSD-95及MAP-2、TAU蛋白表达。采用免疫组织化学染色法检测海马CA1区微管结合蛋白的变化。 结果:①新奇事物识别实验:训练阶段各组大鼠对两个相同物体的探寻指数无显著性差异,24h后进行测试,自主运动组、强迫运动组和功能性电刺激组新奇事物认知指数与模型组比较,差异均具有显著性(P<0.05),自主运动组新奇事物认知指数与强迫运动组、功能性电刺激组比较,差异均有显著性(P<0.05),而功能性电刺激组与强迫运动组比较无显著性差异。②海马区SYN、SYP、PSD-95、MAP-2、TAU蛋白表达水平:自主运动组、强迫运动组和功能性电刺激组SYN、PSD-95、MAP-2、TAU蛋白表达水平均明显高于模型组(P<0.05),功能性电刺激组、自主运动组和强迫运动组两两比较无显著性差异;上述5组SYP蛋白表达组间无显著性差异。 结论:自主运动、强迫运动和功能性电刺激诱导的运动均可促进VD大鼠学习记忆能力的恢复,其可能机制为运动训练促进海马区SYN、PSD-95、MAP-2、TAU蛋白表达,改善海马区突触可塑性。
关键词:血管性痴呆  自主运动  强迫运动  功能性电刺激  突触可塑性  学习  记忆
The effect of different types of exercise training on the ability of learning and memory in vascular dementia rats by improving synaptic plasticity in hippocampus    Download Fulltext
Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou, 510120
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Abstract:
      Abstract Objective:To observe the effects of voluntary exercise, forced exercise and involuntary exercise through functional electrical stimulation on cognitive function and explore the influence of three types of exercise training on plasticity in hippocampus. Method:Male Wistar rats(SPF grade), weighting 250—300g, were anaesthetized with 10% chloral hydrate(300mg/kg), permanent ligation of bilateral common carotid artery(2-VO) was produced. After the model was successfully established, rats received 3 days accommodation (rats that failed to run 270m daily in the wheel were excluded) and randomly assigned to the sham(n=8) and four 2-VO groups (n=32): ①Control vascular dementia group (VD, n = 8), ②Voluntary exercise group (V-Ex, n=8),③Forced wheel exercise group (F-Ex, n= 8) , ④Involuntary exercise group (I-Ex, n=8). After 2-VO, rats in model group and sham-operation group can freely exercise in a cage without any treatment. A week after surgery, V-Ex rats were free to run 270m a day on a running wheel (diameter: 31.8 cm, thickness: 10cm, freely rotates against a resistance of 100g). F-EX rats were forced to run 270m a day on an electric wheel (diameter: 31.8cm, length:40cm) at a speed of 9m/min. I-EX rats were stimulated for 30 minutes every day. The stimulation pattern imitated the gait pattern of forelimb running at the speed of 9m/min. After 14 days, learning and memory test was performed by means of novel object recognition test. Western-blotting technique was used to detect the SYN,SYP,PSD-95,MAP-2 and Tau expression in the hippocampus. Immunohistochemical staining was used to detect the MAP-2 expression in the. hippocampal CA1 zone. Result:①Novel object recognition test:In the training session, all rats showed no significant preference for two familiar objects. In the testing session (24h), novel object recognition indexes in V-Ex, F-Ex and I-Ex groups were also significantly higher than those in VD group. Novel object recognition index in V-Ex group was significantly higher than those in I-Ex and F-Ex groups. However, there was no significant difference between I-Ex and F-Ex groups. ②SYN,SYP,PSD-95,MAP-2 and Tau expression in hippocampus. The expression levels of SYN,PSD-95,MAP-2 and Tau in V-Ex, F-Ex and I-Ex groups were significantly higher than those in VD group. However, there were no significant differences among three groups. The SYP expressed in each group showed no statistical differences. Conclusion:Voluntary exercise, forced exercise and involuntary exercise through functional electrical stimulation can equally improve the ability of learning and memory in vascular dementia rats by improving the expression levels of SYN,PSD-95,MAP-2 and TAU and inducing plasticity in hippocampus.
Keywords:vascular dementia  voluntary exercise  forced exercise  functional electrical stimulation  synaptic plasticity  learning  memory
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