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高前进,柏海平,王彦伟.有氧运动对糖尿病大鼠自噬性肌萎缩的影响[J].中国康复医学杂志,2016,(7):739~745
有氧运动对糖尿病大鼠自噬性肌萎缩的影响    点此下载全文
高前进  柏海平  王彦伟
石家庄学院体育学院运动人体科学实验中心,石家庄,050035
基金项目:石家庄学院科研平台启动基金项目(xJpT005)
DOI:
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摘要:
      摘要 目的:通过观察有氧运动对糖尿病大鼠自噬作用引起的肌萎缩的影响,探讨高血糖和胰岛素缺乏引起肌肉量减少的机制及运动的防治作用。 方法:成年雄性Wistar大鼠采用腹膜内注射链脲菌素的方法诱导糖尿病,然后随机分为糖尿病对照组(diabetic control, DCG),糖尿病运动组(diabetic exercise group,DEG),同时设立正常对照组(control group, CG)和正常运动组(control exercise group, CEG)。有氧运动采用跑台跑训练,采用中等强度每天1h,每周5天共训练4周,4周后所有大鼠测量体重并进行糖耐量测试,取腓肠肌称重,使用组织化学法检测腓肠肌肌肉降解的变化,使用蛋白免疫印迹法检测自噬相关蛋白LC3和肌动蛋白actin变化。 结果:糖尿病运动组(DEG)与糖尿病对照组(DCG)比较,DEG血糖水平显著降低(276.91±18.69 mg/dl vs. 310.75±16.54 mg/dl,P<0.05),体重和腓肠肌重量显著增加(体重303±17.87g vs. 185.44±20.27g,P<0.05;肌重1.80±0.29g vs.0.92±0.20g,P<0.05)。DEG的自噬性蛋白LC3表达水平比DCG显著降低(225±27 vs.435±31)。 结论:由于糖尿病过度的分解代谢激活了过度自噬作用,导致骨骼肌肌萎缩。4周跑台训练提高了糖尿病大鼠的肌肉量,说明有氧运动通过抑制自噬作用,可以防治糖尿病患者的骨骼肌肌萎缩。
关键词:有氧运动  糖尿病  大鼠  自噬作用  肌萎缩
Effects of aerobic exercise on autophagy-induced muscle atrophy in diabetic rats    Download Fulltext
Human Movement Science Experimental Center of Shijiazhuang College’ Sport College, Shijiazhuang,050035
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Abstract:
      Abstract Objective: To investigate the mechanism by which hyperglycemia and insulin deficiency decrease muscle mass, and the exercise therapeutic effect through observing the effect of aerobic exercise training on autophagy-induced muscle atrophy. Method: Adult male Wistar rats were injected intraperitoneally with Streptozotocin (60mg/kg body weight) to induce diabetes. All rats were divided into four groups randomly: diabetic control group(DCG), diabetic exercise group(DEG), normal control group(CG) and normal control exercise group(CEG). Aerobic exercise training was performed on a treadmill at a low-moderate intensity(50%—60% of the initial maximum speed), 1 hour per day, 5 days per week. After 4 weeks, we performed intraperitonal glucose test and measured body weight and gastrocnemius muscle mass. The protein degradation and the level of autophagy were examined by histochemistry. At the same time, we also examined the level of LC3 and actin in isolated gastrocnemius by western blot analysis. Result:The body weight and gastrocnemius muscle mass were higher in the diabetic exercise group rats than that in the diabetic control group rats(303±17.87g vs. 185.44±20.27g,P<0.05;muscle weight 1.80±0.29g vs.0.92±0.20g,P<0.05). Compared with the diabetic control group, the diabetic exercise group had the lower blood glucose level(276.91±18.69 mg/dl vs. 310.75±16.54 mg/dl,P<0.05), the increased intracellular contractile protein expression,and the decreased autophagic protein expression(225±27 vs.435±31). Conclusion: Four weeks treadmill running improves the muscle mass in the diabetes-induced skeletal muscle atrophy, suggesting the activation of autophagy in diabetes contributes to muscle atrophy through hypercatablic metabolism. Moreover, that aerobic exercise modifies or reverses skeletal muscle atrophy may be mediated by suppressing autophagy in diabetic patients.
Keywords:aerobic exercise training  diabetes  rat  autophagy  muscle atrophy
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