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冯晓东,高玲莉,李瑞青,孙伟娟,白俊敏.电针对脑缺血再灌注模型大鼠脑组织Beclin-1蛋白及基因表达的影响[J].中国康复医学杂志,2016,(12):1307~1310
电针对脑缺血再灌注模型大鼠脑组织Beclin-1蛋白及基因表达的影响    点此下载全文
冯晓东  高玲莉  李瑞青  孙伟娟  白俊敏
河南中医学院第一附属医院,郑州,450000
基金项目:河南省教育厅科学技术研究重点项目(13A360598); 河南省中医临床学科领军人才培育计划资助项目(2100202)
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摘要:
      摘要 目的:观察电针(EA)对脑缺血再灌注认知障碍模型大鼠脑组织中Beclin-1的影响,探讨脑卒中后认知障碍的康复机制。 方法:健康SD雄性大鼠105只,随机分为3组:电针组、模型组和假手术组,每组35只;大鼠缺血再灌注模型采用改良线栓法栓塞左侧大脑中动脉制备。电针“神庭穴”、“百会穴”各30min,1次/日,从术后第1天至第10天动物处死。采用Morris水迷宫实验观测模型大鼠的学习、记忆能力;采用Western blot和荧光定量PCR法检测大鼠脑组织Beclin-1蛋白和基因的表达。 结果:造模后的第4到第8天,模型组大鼠的逃避潜伏期较假手术组长(P<0.05),EA组大鼠的逃避潜伏期较模型组缩短(P<0.05)。电针组大鼠海马及左侧大脑皮质中Beclin-1蛋白的电泳条带与假手术组相比,电针组条带灰度值较假手术组高(P<0.05),与模型组相比,电针组Beclin-1蛋白电泳条带灰度较模型组高(P<0.05)。模型组和电针组的Beclin-1基因表达的2-△△Ct与假手术组相比差异明显,均升高(P<0.05)。但电针组Beclin-1基因表达的2-△△Ct明显较模型组高(P<0.05) 结论:①电针神庭、百会可以促进脑缺血再灌注后认知障碍模型大鼠的认知功能的恢复;②电针可以提高缺血再灌注模型大鼠脑组织中的Beclin-1表达。Beclin-1是一种自噬相关蛋白,直接执行自噬的过程,被作为自噬发生的标志物。对自噬网络系统的调控可能是电针能够改善脑卒中后认知障碍的生物学机制之一。
关键词:电针  认知障碍  神庭  百会  Beclin-1  脑缺血
Effects of electroacupuncture on expression of Beclin-1 in cerebral ischemia rats    Download Fulltext
The First Affiliated Hospital of Henan College of TCM, Zhengzhou, 450008
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Abstract:
      Abstract Objective: To observe effects of electroacupuncture (EA) on expression of Beclin-1 in the brain of cognitive disorder model rats after cerebral ischemia reperfusion and to explore the mechanism of EA treatment for cognitive disorder. Method: Total of 105 SD male rats were randomized into 3 groups:electroacupuncture treatment group, model group and sham operation group, 35 rats in each.The model group and electroacupuncture group were treated with MCAO to produce cerebral ischemia reperfusion cognitive impairment model rats. We applied EA at Shenting (DU24) and Baihui (GV20) 30min once daily, from the day after operation to sacrificed at day 10. The learning and memory ability was tested by Morris water maze and the changes of Beclin-1 in left cortex were examined by RT-PCR and Western blot. Result: From 4th to 8th day after modeling, the latency of water maze in model group is longer than sham operation group (P<0.05). Comparing with the model group, the latency of water maze decreased in electroacupuncture group (P<0.05) .The grey scale value of Beclin-1 protein of electrophoresis bands obtained from left cerebral cortex and hippocampus of rats is higher in electroacupuncture group than in model group(P<0.05), in model group is higher than in sham operation group (P<0.05). Comparing with sham operation group,2-△△Ct of Beclin-1 gene expression is obvious higher both in model group and EA group (P<0.05) , EA group is higher than model group(P<0.05). Conclusion: EA at "Shenting", "Baihui" could ameliorate the cognitive disorder of cerebral ischemia rats and enhance expression of Beclin-1 in brain of model rats. Beclin-1 is one of the autophagy-related protein executing the process of autophagy directly,It accepts many signals to regulate autophagy process,and has been used as autophagy marker to test autophagy. Up regulation of Beclin-1 may be one of the biological mechanism of EA treatment for cognitive impairment after cerebral stroke.
Keywords:electroacupuncture  cognitive impairment  Shenting  Baihui  Beclin-1  cerebral ischemia
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